Reverse transcriptase PCR - RT-PCR
RT-PCR is a technique which makes it possible to make a PCR (polymerase chain reaction) from an RNA sample. RNA is first retrotranscribed by an enzyme called reverse transcriptase, which allows the synthesis of the complementary DNA (cDNA). The latter is then used to carry out a PCR. Reverse transcriptase or reverse transcriptase (RT) is an enzyme used by retroviruses and retrotransposons that transcribe the genetic information of viruses or retrotransposons from RNA to DNA, which can integrate into the genome of the host.
RT-PCR has been developed to use RNAs as a PCR amplification matrix. It is certainly the most sensitive method for detecting (and possibly quantifying) messenger RNAs at the level of an organ, tissue or cell. For this purpose, it is often carried out in situ (PCR in situ), ie on fixed biological material. It is also used for the construction of cDNA libraries, sorting of mRNA (Differencial Display RT-PCR) as well as the construction of DNA probes.
Frequently Asked Questions (FAQ)
Where can I find rt-pcr products on the CliniSciences website?
rt-pcr products are available in this section and can be sorted by product families or sub-categories, such as Reverse transcriptase, RT-PCR Mixes and Kits. You can browse the catalogue tree or use the search bar with a product name, catalogue reference, brand or supplier.
What types of products are available in the rt-pcr category?
The rt-pcr category brings together various product references, consumables, reagents, kits, instruments or laboratory solutions, depending on the relevant product family. The RT-PCR Mixes and Kits, Reverse transcriptase sub-categories allow you to find the products that meet your needs more quickly.
How can I quickly find a rt-pcr product?
To find a rt-pcr product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does CliniSciences stock several product codes in the rt-pcr category?
Yes, CliniSciences distributes several product references in the rt-pcr category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for rt-pcr products?
Yes, you can request a quote for rt-pcr products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a rt-pcr product is available to order?
The availability of a rt-pcr product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.
What should I do if I cannot find the rt-pcr product I am looking for?
If the rt-pcr product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all rt-pcr products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.