Reagents and instruments for immunology, cell biology and molecular biology.
 
Citrate Synthase Assay Kit

Citrate Synthase Assay Kit

Citrate synthase assay kits are specialized tools designed to measure the activity of citrate synthase, a key enzyme in the Krebs cycle (tricarboxylic acid cycle, TCA cycle). Citrate synthase catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate, marking the first step of this crucial metabolic pathway. These kits are widely used in mitochondrial function studies, metabolic research, and clinical investigations.

Principle of Citrate Synthase Assay Kits

Citrate synthase activity is typically measured using colorimetric or fluorometric methods:

  • Colorimetric Assays: Citrate synthase catalyzes the reaction between acetyl-CoA and oxaloacetate, producing citrate and CoA-SH. CoA-SH reacts with DTNB (5,5'-dithiobis-(2-nitrobenzoic acid)) to produce TNB (5-thio-2-nitrobenzoic acid), which absorbs light at 412 nm. The rate of color development is proportional to enzyme activity.
  • Fluorometric Assays: Citrate synthase activity generates fluorescent products detectable at specific excitation/emission wavelengths.

Applications

Citrate synthase assay kits are used in various fields:

  • Mitochondrial Function Studies: Citrate synthase is an exclusive marker of mitochondrial integrity and activity. Its measurement aids in assessing mitochondrial health.
  • Metabolic Research: These kits help analyze citrate synthase activity in tissues and cells to understand metabolic disorders such as obesity and diabetes.
  • Drug Development: Evaluating the effects of drugs on mitochondrial enzymes provides insights into therapeutic efficacy and toxicity.
  • Cellular Bioenergetics: Used to study energy production pathways in different cell types, including cancer cells.

Key Features

Citrate synthase assay kits offer several advantages:

  • High Sensitivity: Detects enzyme activity as low as 1 mU, enabling precise measurements even in small samples.
  • Versatility: Compatible with various sample types, including tissue homogenates (liver, kidney, brain), cell lysates (adherent or suspension cells), and isolated mitochondria.
  • Automation-Ready: Designed for use with microplate readers for high-throughput applications.
  • Rapid Protocols: Assay times typically range from 30 minutes to 1 hour.

Citrate synthase assay kits are indispensable tools for studying mitochondrial function and energy metabolism. Their sensitivity, versatility, and ease of use make them ideal for applications ranging from basic biochemical research to clinical investigations. By enabling precise measurement of citrate synthase activity, these kits contribute significantly to advancing our understanding of cellular bioenergetics and metabolic regulation.

Frequently Asked Questions (FAQ)

How can I quickly find a citrate synthase product?

To find a citrate synthase product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does CliniSciences stock several product codes in the citrate synthase category?

Yes, CliniSciences distributes several product references in the citrate synthase category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for citrate synthase products?

Yes, you can request a quote for citrate synthase products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a citrate synthase product is available to order?

The availability of a citrate synthase product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.

What should I do if I cannot find the citrate synthase product I am looking for?

If the citrate synthase product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all citrate synthase products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.

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