Human whole blood is a complex biological matrix comprising plasma, erythrocytes, leukocytes, platelets, and circulating molecular components, including proteins, metabolites, nucleic acids, and extracellular vesicles. Peripheral venous blood collected in K₂EDTA (dipotassium ethylenediaminetetraacetic acid) is widely used in biomedical and translational research. K₂EDTA acts primarily by chelating ionized calcium required for the coagulation cascade, thereby maintaining blood in an anticoagulated state. Under appropriate collection, handling, and storage conditions, K₂EDTA whole blood provides cellular and molecular material for a range of downstream applications.

Key Features

• Anticoagulated Whole Blood – Calcium chelation prevents clot formation while maintaining a heterogeneous blood-cell population.

• Native Cellular Matrix – Contains circulating erythrocytes, leukocytes, and platelets, supporting hematological and immune-cell analyses.

• Molecular Research Material – Provides a source of genomic DNA and other cellular or circulating molecular components for selected molecular and epigenetic studies.

• Versatile Fractionation – Depending on the experimental objective, samples can be processed to obtain plasma, buffy coat, erythrocyte-enriched fractions, or PBMCs, commonly using density-gradient methods for PBMC isolation.

Usual Research Uses

• Genomic DNA Extraction, PCR, and qPCR.

• Hematology and Immune-Cell Profiling.

• PBMC Isolation and Downstream Cellular Assays.

• Genetic and Epigenetic Research.

• Circulating Biomarker and Molecular Studies.

• Flow Cytometry and Characterization of Circulating Leukocyte Populations.