NGS Transcriptome sequencing - Library preparation
The term RNA-Seq or RNA-Seq refers to a next-generation sequencing approach (NGS) that provides a snapshot of the entire transcriptome or messenger RNA (mRNA) profile at a given point in time.
Whole transcriptome analysis aims at capturing coding and non-coding RNA and quantifying the heterogeneity of gene expression in cells, tissues, organs and even the entire body.
The sequencing of the whole transcriptome therefore provides the most complete view possible. It is the method that allows the sequencing of all transcripts of coding and non-coding RNA. In other words, the messenger RNAs that code for proteins but also all other micro RNAs, small interfering RNAs, extracellular RNAs.
The other advantage of whole genome sequencing is that the extraction and purification of the total RNA is easier and cheaper than messenger RNA for example. However, it is necessary to remove the ribosomal RNA that is present in too large quantities and that could interfere with other types of RNA. This is called ribosomal RNA depletion.
Frequently Asked Questions (FAQ)
Where can I find whole transcriptome products on the CliniSciences website?
What types of products are available in the whole transcriptome category?
How can I quickly find a whole transcriptome product?
To find a whole transcriptome product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.
Does CliniSciences stock several product codes in the whole transcriptome category?
Yes, CliniSciences distributes several product references in the whole transcriptome category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.
Can I request a quote for whole transcriptome products?
Yes, you can request a quote for whole transcriptome products from the product page or by contacting CliniSciences (info@clinisciences.com). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.
How can I find out if a whole transcriptome product is available to order?
The availability of a whole transcriptome product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact CliniSciences with the product code or name of the product you are looking for.
What should I do if I cannot find the whole transcriptome product I am looking for?
If the whole transcriptome product you are looking for does not appear in the catalogue, please contact CliniSciences with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.
Are all whole transcriptome products available under the same terms and conditions?
No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.