Vimentin (VIM) pSer71 Rat Monoclonal Antibody [Clone ID: TM71]
CAT#: AM26458AF-N
Vimentin (VIM) pSer71 rat monoclonal antibody, clone TM71, Azide Free
Specifications
Product Data | |
Clone Name | TM71 |
Applications | IF, WB |
Recommended Dilution | Western blot: 1 μg/ml for chemiluminescence detection system. Immunocytochemistry: 1 μg/ml. For details see protocol below. |
Reactivities | Human, Mouse, Rat |
Host | Rat |
Isotype | IgG2a |
Clonality | Monoclonal |
Immunogen | KLH conjugated phospho-peptide PV71 |
Specificity | This antibody recognizes the site-specific phosphorylation of vimentin at Ser71. |
Formulation | PBS containing 50% glycerol, pH 7.2. No preservative is contained. State: Azide Free State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein G agarose |
Conjugation | Unconjugated |
Storage | Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Database Link | |
Background | Components of intermediate filaments provide information on the origin of vertebrate cells. Antibody to vimentin can be used as to identify the vimentin subclass of intermediate filaments. Vimentin is a ~58 kDa, widely expressed protein that thought to be involved mainly in structural processes, such as wound healing. Scientists have also recently determined that activated human macrophages secrete vimentin into the extracellular space, and overproduction of vimentin has been associated with cellular senescence. |
Synonyms | VIM |
Note | This product was originally produced by MBL International. Protocol: SDS-PAGE & Western Blotting |
Reference Data |
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