Anti-APPL2 antibody (1-300)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	APPL2
Gene ID:	55198
Uniprot ID:	DP13B_HUMAN
Immunogen Region:	1-300
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human APPL2 (NP_060641.2).
Immunogen Sequence:	MPAVDKLLLEEALQDSPQTR SLLSVFEEDAGTLTDYTNQL LQAMQRVYGAQNEMCLATQQ LSKQLLAYEKQNFALGKGDE EVISTLHYFSKVVDELNLLH TELAKQLADTMVLPIIQFRE KDLTEVSTLKDLFGLASNEH DLSMAKYSRLPKKKENEKVK TEVGKEVAAARRKQHLSSLQ YYCALNALQYRKQMAMMEPM IGFAHGQINFFKKGAEMFSK RMDSFLSSVADMVQSIQVE

Tissue Specificity	High levels in brain, heart, kidney and skeletal muscle.
Function	Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Plays a role in immune response by modulating phagocytosis, inflammatory and innate immune responses. In macrophages, enhances Fc-gamma receptor-mediated phagocytosis through interaction with RAB31 leading to activation of PI3K/Akt signaling. In response to LPS, modulates inflammatory responses by playing a key role on the regulation of TLR4 signaling and in the nuclear translocation of RELA/NF-kappa-B p65 and the secretion of pro- and anti-inflammatory cytokines. Also functions as a negative regulator of innate immune response via inhibition of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Plays a role in endosomal trafficking of TGFBR1 from the endosomes to the nucleus. Plays a role in cell metabolism by regulating adiponecting ans insulin signaling pathways and adaptative thermogenesis. In muscle, negatively regulates adiponectin-simulated glucose uptake and fatty acid oxidation by inhibiting adiponectin signaling pathway through APPL1 sequestration thereby antagonizing APPL1 action. In muscles, negativeliy regulates insulin-induced plasma membrane recruitment of GLUT4 and glucose uptake through interaction with TBC1D1. Plays a role in cold and diet-induced adaptive thermogenesis by activating ventromedial hypothalamus (VMH) neurons throught AMPK inhibition which enhances sympathetic outflow to subcutaneous white adipose tissue (sWAT), sWAT beiging and cold tolerance. Also plays a role in other signaling pathways namely Wnt/beta-catenin, HGF and glucocorticoid receptor signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex. May affect adult neurogenesis in hippocampus and olfactory system via regulating the sensitivity of glucocorticoid receptor. Required for fibroblast migration through HGF cell signaling.
Protein Name	Dcc-Interacting Protein 13-Beta Dip13-Beta Adapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 2
Cellular Localisation	Early Endosome Membrane Peripheral Membrane Protein Nucleus Cell Membrane Endosome Membrane Cytoplasm Cytoplasmic Vesicle Phagosome Cell Projection Ruffle Ruffle Membrane Phagosome Membrane Early Endosomal Membrane-Bound And Nuclear Translocated Into The Nucleus Upon Release From Endosomal Membranes Following Internalization Of Egf Associates Dynamically With Cytoplasmic Membrane Structures That Undergo Changes In Shape Movement Fusion And Fission Events Pi(4 5)P2 Levels Are Important For Membrane Association Of Appl2 Absent Of Endosome In Macrophage Colocalized With Rab31 At Early-Stage Phagosome Localized On Macropinosomes In Lps-Activated Macrophages Associated With Membrane Domains In Contact With Pathogens And Pathogen-Derived Ligands Like Lps First Recruited To The Ruffles And Accumulates On Macropinosomes
Alternative Antibody Names	Anti-Dcc-Interacting Protein 13-Beta antibody Anti-Dip13-Beta antibody Anti-Adapter Protein Containing Ph Domain - Ptb Domain And Leucine Zipper Motif 2 antibody Anti-APPL2 antibody Anti-DIP13B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance