Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CaM Kinase II (Thr286) Antibody

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Cat# p1005-286t

Size : 25uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Phosphosolutions

Contact local distributor :

Phone : +1 850 650 7790

Anti-CaM Kinase II (Thr286) Antibody

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Our Anti-CaM Kinase II (Thr286) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, rabbit, and rat CaM Kinase II (Thr286) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring CaMKIIα WT and F89G phosphorylation of T286 (cat. p1005-286, 1:2500). Image from publication CC-BY-4.0. PMID: 37648853

Representative immunoblot and quantification of in vitro kinase reactions at 30 °C in 1 mM ATP measuring AS283-mediated inhibition of CaMKII phosphorylation of GluA1 S831 (pS831) (cat. p1160-831, 1:2000) and autophosphorylation of CaMKII T286 (pT286) (cat. p1005-286, 1:2500) with varying concentrations of AS283 (0.41–10 μM). For a negative control (Neg.), ATP was omitted. Image from publication CC-BY-4.0. PMID: 37648853

Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).