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> Anti-ERp57 Antibody (3R372)

Anti-ERp57 Antibody (3R372)

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Cat# NB-64-155636-100uL

Size : 100uL

Brand : Neo Biotech


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Anti-ERp57 Antibody (3R372)

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Synonyms:

Catalog No. TMAB-05686 Copy Product Info
Anti-ERp57 Antibody (3R372) is a Rabbit antibody targeting ERp57. Anti-ERp57 Antibody (3R372) can be used in WB,IHC-P,IHC-Fr,IP,IF.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Description
Anti-ERp57 Antibody (3R372) is a Rabbit antibody targeting ERp57. Anti-ERp57 Antibody (3R372) can be used in WB,IHC-P,IHC-Fr,IP,IF.
Ig Type
IgG
Clone
3R372
Reactivity
Human
Verified Activity
1. 25 μg total protein per lane of various lysates (see on figure) probed with ERp57 monoclonal antibody, unconjugated (TMAB-05686) at 1: 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp57 Monoclonal Antibody, Unconjugated (TMAB-05686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp57 Monoclonal Antibody, Unconjugated (TMAB-05686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp57 Monoclonal Antibody, Unconjugated (TMAB-05686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp57 Monoclonal Antibody, Unconjugated (TMAB-05686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp57 Monoclonal Antibody, Unconjugated (TMAB-05686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with ERp57 Monoclonal Antibody, Unconjugated (TMAB-05686) at 1: 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG H&L Secondary Antibody-HRP and DAB staining.
Application
WB
IHC-P
IHC-Fr
IP
IF
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IP: 1:20-50; IF: 1:100-500
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue SpecificityDetected in the flagellum and head region of spermatozoa (at protein level).
ConstructionRecombinant Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation10mM phosphate buffered saline (pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Research BackgroundProtein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone.
Antigen Details
Immunogen
A synthesized peptide: human ERp57
Antigen Species
Human
Gene Name
PDIA3
Gene ID
2923
Protein Name
Protein disulfide-isomerase A3
Uniprot ID
P30101
Chemical Properties
Molecular WeightTheoretical: 57 kDa. Actual: 57 kDa.
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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