General Info
| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-FZD8 (130-275) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | FZD8 |
| Gene ID: | 8325 |
| Uniprot ID: | FZD8_HUMAN |
| Immunogen Region: | 130-275 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-275 of human FZD8 (NP_114072.1). |
| Immunogen Sequence: | PDRMRCDRLPEQGNPDTLCM DYNRTDLTTAAPSPPRRLPP PPPGEQPPSGSGHGRPPGAR PPHRGGGRGGGGGDAAAPPA RGGGGGGKARPPGGGAAPCE PGCQCRAPMVSVSSERHPLY NRVKTGQIANCALPCHNPFF SQDERA |
Description
| Tissue Specificity | Most abundant in fetal kidney, followed by brain and lung. In adult tissues, expressed in kidney, heart, pancreas and skeletal muscle. |
| Post Translational Modifications | Ubiquitinated by ZNRF3, leading to its degradation by the proteasome. |
| Function | Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. |
| Protein Name | Frizzled-8Fz-8Hfz8 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-4608870Reactome: R-HSA-4641263Reactome: R-HSA-5340588 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinGolgi ApparatusCell MembraneColocalizes With Gopc At The Golgi Apparatus |
| Alternative Antibody Names | Anti-Frizzled-8 antibodyAnti-Fz-8 antibodyAnti-Hfz8 antibodyAnti-FZD8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance