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Anti-GATA3 Polyclonal Antibody

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Cat# NB-64-113320-100uL

Size : 100uL

Brand : Neo Biotech


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Anti-GATA3 Polyclonal Antibody

(Synonyms: Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA 3) Copy Product Info

Synonyms: Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA 3

Catalog No. TMAB-00748 Copy Product Info
Anti-GATA3 Polyclonal Antibody is a Rabbit antibody targeting GATA3. Anti-GATA3 Polyclonal Antibody can be used in FCM,IF-Tissue,IHC-Fr,IHC-P.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-GATA3 Polyclonal Antibody is a Rabbit antibody targeting GATA3. Anti-GATA3 Polyclonal Antibody can be used in FCM,IF-Tissue,IHC-Fr,IHC-P.
SynonymsTrans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA 3
Ig Type
IgG
Reactivity
Human (predicted:Mouse,Rat,Chicken,Dog,Pig,Cow,GuineaPig)
Verified Activity
1. Tissue/cell: human meningioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-GATA3 Polyclonal Antibody, Unconjugated (TMAB-00748) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-GATA3 antibody (TMAB-00748)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Anti-GATA3 Polyclonal Antibody,Anti-GATA3 Polyclonal Antibody
Application
FCM
IF-Tissue
IHC-Fr
IHC-P
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF-Tissue:1:100-500; FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationNucleus.
Tissue SpecificityT-cells and endothelial cells.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundMembers of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human GATA3
Antigen Species
Human
Gene Name
GATA3
Gene ID
2625
Protein Name
Trans-acting T-cell-specific transcription factor GATA-3
Uniprot ID
P23771
Biology Area
Ectoderm,ChIP antibodies,Zinc Finger,Non-CD,Ectoderm
Function
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Chemical Properties
Molecular WeightAntigen Theoretical: 49 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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