Anti-Mek1 (MAPK/ERK Kinase 1, MEK 1, MEKK1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, Map2k1, MAPKK 1, MAPKK1, Prkmk1, ERK Activator Kinase 1) (Biotin) Monoclonal Antibody
Cat# M2865-15-Biotin-100ul
Size : 100ul
Brand : US Biological
M2865-15-Biotin Mek1 (MAPK/ERK Kinase 1, MEK 1, MEKK1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, Map2k1, MAPKK 1, MAPKK1, Prkmk1, ERK Activator Kinase 1) (Biotin)
Clone Type
PolyclonalHost
mouseSource
mouseIsotype
IgG1Grade
PurifiedApplications
IP WBCrossreactivity
Hu Mo Rt XeShipping Temp
Blue IceStorage Temp
-20°CMEK (MAP kinase or ERK kinase) is also known as MAP kinase kinase (MAPKK). MEK1 is a dual tyrosine/threonine kinase that phosphorylates and activates MAP kinase (ERK1 and ERK2). MEK1 has a molecular weight of 43.5kD and contains 393 amino acids. MEK1 is phosphorylated and activated by serine/threonine kinases via 2 different pathways(3). These kinases include; c-raf-1, v-raf-1, c-Mos, and MEKK. MEKK shares homology with yeast kinases Ste11 and byr2. MEK2 has been identified and cloned(4). MEK2 differs from MEK1 in the amino terminal region and between the conserved kinase subdomains 9 and 10. The MEK1 murine gene is found on chromosome 9 the MEK2 gene on chromosome 10. It is likely that more members of the MEK family exist.
Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2000
Immunoprecipitation: 2-5ug
Optimal dilutions to be determined by the researcher.
Positive Control:
Mouse brain cell lysates
Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.