Anti-Mek1 (MAPK/ERK Kinase 1, MEK 1, MEKK1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, Map2k1, MAPKK 1, MAPKK1, Prkmk1, ERK Activator Kinase 1) (Biotin) Monoclonal Antibody

Cat# M2865-15-Biotin-100ul

Size : 100ul

Brand : US Biological

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M2865-15-Biotin Mek1 (MAPK/ERK Kinase 1, MEK 1, MEKK1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, Map2k1, MAPKK 1, MAPKK1, Prkmk1, ERK Activator Kinase 1) (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
mouse
Isotype
IgG1
Grade
Purified
Applications
IP WB
Crossreactivity
Hu Mo Rt Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C

MEK (MAP kinase or ERK kinase) is also known as MAP kinase kinase (MAPKK). MEK1 is a dual tyrosine/threonine kinase that phosphorylates and activates MAP kinase (ERK1 and ERK2). MEK1 has a molecular weight of 43.5kD and contains 393 amino acids. MEK1 is phosphorylated and activated by serine/threonine kinases via 2 different pathways(3). These kinases include; c-raf-1, v-raf-1, c-Mos, and MEKK. MEKK shares homology with yeast kinases Ste11 and byr2. MEK2 has been identified and cloned(4). MEK2 differs from MEK1 in the amino terminal region and between the conserved kinase subdomains 9 and 10. The MEK1 murine gene is found on chromosome 9 the MEK2 gene on chromosome 10. It is likely that more members of the MEK family exist.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Immunoprecipitation: 2-5ug
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse brain cell lysates

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3G188|Host: mouse|Source: mouse|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.|Purity: Purified|Immunogen: Purified MEK from mouse T cells and recombinant MEK1.|Specificity: Recognies mouse MEK1. Does not crossreact with MEK2. Species crossreactivity: Human, rat, Xenopus.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Purified MEK from mouse T cells and recombinant MEK1.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Purity
Purified
Specificity
Recognies mouse MEK1. Does not crossreact with MEK2. Species crossreactivity: Human, rat, Xenopus.
References
1. Crews, C.M. and Erikson, R.L.; Proc Natl Acad Sci 89:8205–8209 (1992). 2. Crews, C.M., Alessandrini, A., and Erikson, R.L; The Primary Structure of MEK, a Protein Kinase That Phosphorylates the ERK Gene Product, Science 258:478–480 (1992). 3. Crews, C.M. and Erikson, R.L.; Extracellular Signals and Reversible Protein Phosphorylation: What to Mek of It All, Cell 74:215–217 (1993). 4. Broitt, B.K. et al; MEK2 is a kinase related to MEK1 and is differentially expressed in murine tissues, Cell Growth and Differentiation (Nov. 1993–In press). 5. Gredlich, H. and Erikson, R.L; J. Biol. Chem. 273:13280–13288 (1998). 6. Broitt, B. K. et al; Cell Growth and Differentiation (1993).