General Info
| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-MIOS (556-875) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | MIOS |
| Gene ID: | 54468 |
| Uniprot ID: | MIOS_HUMAN |
| Immunogen Region: | 556-875 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 556-875 of human MIOS (NP_061878.3). |
| Immunogen Sequence: | VVAMALSGYTDEKNSLWREM CSTLRLQLNNPYLCVMFAFL TSETGSYDGVLYENKVAVRD RVAFACKFLSDTQLNRYIEK LTNEMKEAGNLEGILLTGLT KDGVDLMESYVDRTGDVQTA SYCMLQGSPLDVLKDERVQY WIENYRNLLDAWRFWHKRAE FDIHRSKLDPSSKPLAQVFV SCNFCGKSISYSCSAVPHQG RGFSQYGVSGSPTKSKVTSC PGCRKPLPRCALCLINMGT |
Description
| Function | As a component of the GATOR2 complex, functions as an activator of the amino acid-sensing branch of the mTORC1 signaling pathway. The GATOR2 complex indirectly activates mTORC1 through the inhibition of the GATOR1 subcomplex. GATOR2 probably acts as a E3 ubiquitin-protein ligase toward GATOR1. In the presence of abundant amino acids, the GATOR2 complex mediates ubiquitination of the NPRL2 core component of the GATOR1 complex, leading to GATOR1 inactivation. In the absence of amino acids, GATOR2 is inhibited, activating the GATOR1 complex. Within the GATOR2 complex, MIOS is required to prevent autoubiquitination of WDR24, the catalytic subunit of the complex. The GATOR2 complex is required for brain myelination. |
| Protein Name | Gator2 Complex Protein MiosMissing Oocyte Meiosis Regulator Homolog |
| Database Links | Reactome: R-HSA-9639288 |
| Cellular Localisation | Lysosome Membrane |
| Alternative Antibody Names | Anti-Gator2 Complex Protein Mios antibodyAnti-Missing Oocyte Meiosis Regulator Homolog antibodyAnti-MIOS antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance