Anti-PRKCI antibody

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:2000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PRKCI
Gene ID:	5584
Uniprot ID:	KPCI_HUMAN
Immunogen Region:	1-596
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-596 of human PRKCI (P41743).
Immunogen Sequence:	MPTQRDSSTMSHTVAGGGSG DHSHQVRVKAYYRGDIMITH FEPSISFEGLCNEVRDMCSF DNEQLFTMKWIDEEGDPCTV SSQLELEEAFRLYELNKDSE LLIHVFPCVPERPGMPCPGE DKSIYRRGARRWRKLYCANG HTFQAKRFNRRAHCAICTDR IWGLGRQGYKCINCKLLVHK KCHKLVTIECGRHSLPQEPV MPMDQSSMHSDHAQTVIPYN PSSHESLDQVGEEKEAMNT

Tissue Specificity	Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
Post Translational Modifications	Phosphorylation at Thr-412 in the activation loop is not mandatory for activation. Upon neuronal growth factor (NGF) stimulation, phosphorylated by SRC at Tyr-265, Tyr-280 and Tyr-334. Phosphorylation at Tyr-265 facilitates binding to KPNB1/importin-beta regulating entry of PRKCI into the nucleus. Phosphorylation on Tyr-334 is important for NF-kappa-B stimulation. Phosphorylated at Thr-564 during the initial phase of long term potentiation.
Function	Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non-small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis. Involved in early synaptic long term potentiation phase in CA1 hippocampal cells and short term memory formation.
Protein Name	Protein Kinase C Iota Type Atypical Protein Kinase C-Lambda/Iota Prkc-Lambda/Iota Apkc-Lambda/Iota Npkc-Iota
Database Links	Reactome: R-HSA-1912408 Reactome: R-HSA-209543 Reactome: R-HSA-420029
Cellular Localisation	Cytoplasm Membrane Endosome Nucleus Transported Into The Endosome Through Interaction With Sqstm1/P62 After Phosphorylation By Src Transported Into The Nucleus Through Interaction With Kpnb1 Colocalizes With Cdk7 In The Cytoplasm And Nucleus Transported To Vesicular Tubular Clusters (Vtcs) Through Interaction With Rab2a
Alternative Antibody Names	Anti-Protein Kinase C Iota Type antibody Anti-Atypical Protein Kinase C-Lambda/Iota antibody Anti-Prkc-Lambda/Iota antibody Anti-Apkc-Lambda/Iota antibody Anti-Npkc-Iota antibody Anti-PRKCI antibody Anti-DXS1179E antibody

Information sourced from Uniprot.org

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