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> Anti-Rat IgM (Peroxidase Conjugated) Secondary Antibody

Anti-Rat IgM (Peroxidase Conjugated) Secondary Antibody

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Cat# ASR2963

Size : 1.5mg

Brand : Abcepta


Anti-Rat IgM (Peroxidase Conjugated) Secondary Antibody

Rabbit Polyclonal, Peroxidase (Horseradish)

 

Product Information

Description Anti-RAT IgM (RABBIT) Antibody Peroxidase Conjugated
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Rat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:60,000
Western Blot 1:1,000-1:5,000
Immunochemistry 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgM µ chain
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgM mu heavy chain
Species of Origin rabbit
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Additional Information

Shipping Condition Ambient
Purity Anti-Rat IgM (mu chain) was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgM and Rat Serum.  No reaction was observed against Rat IgA or Rat IgG.   ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas
  • Secondary Antibodies
  • Peroxidase (Horseradish)
  • Texas Red®