Anti-RFP - Red Fluorescent Protein (clone RF5R)
Cat# AS153028
Size : 50ug
Brand : Agrisera
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Anti-RFP | Red Fluorescent Protein (clone RF5R)
Product no: AS15 3028
AS15 3028 | Clonality: monoclonal | Host: Mouse | Reactivity: Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc.
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| Immunogen: | KLH-conjugated RFP from the Discosoma sp. (sea anemone) N-terminal peptide |
| Sub class: | IgG1 |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Purity: | Total IgG1. Protein A purified in 1mg/mL in 10mM PBS, pH 7.2. Contains 0.05% sodium azide. |
| Format: | Liquid |
| Quantity: | 50 µg |
| Storage: | Store at -20°C for up to 2 years. |
| Tested applications: | Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | 1 : 1000-3000 (WB) |
| Confirmed reactivity: | Native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, etc |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
| Additional information: | Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally |
| Additional information (application): | Antibody is purified on Protein A. Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature. |
| Background: | RFP(Red Fluorescent Protein) is a tag that can be added to a protein of interest as a fusion protein to unable purification and detection. |
| Selected references: | Cecchini et al. (2020). Kinases and protein motifs required for AZI1 plastid localization and trafficking during plant defense induction. Plant J. 2020 Dec 20. doi: 10.1111/tpj.15137. Epub ahead of print. PMID: 33342031. |