General Info
| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-SLC12A3 (1-140) is suitable for use in Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:1000-1:5000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | SLC12A3 |
| Gene ID: | 6559 |
| Uniprot ID: | S12A3_HUMAN |
| Immunogen Region: | 1-140 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human SLC12A3 (NP_000330.2). |
| Immunogen Sequence: | MAELPTTETPGDATLCSGRF TISTLLSSDEPSPPAAYDSS HPSHLTHSSTFCMRTFGYNT IDVVPTYEHYANSTQPGEPR KVRPTLADLHSFLKQEGRHL HALAFDSRPSHEMTDGLVEG EAGTSSEKNPEEPVRFGWVK |
Description
| Tissue Specificity | Predominantly expressed in the kidney (at protein level). Localizes to the distal convoluted tubules (at protein level). Not detected in normal aorta, but abundantly expressed in fatty streaks and advanced atherosclerotic lesions (at protein level). |
| Post Translational Modifications | Ubiquitinated.ubiquitination is essential for regulation of endocytosis. The BCR(KLHL3) complex was initially identified as a candidate ubiquitin ligase for SLC12A3. However, it was later shown that it is not the case. Phosphorylated at Thr-46, Thr-55, Thr-60 and Ser-91 by OXSR1/OSR1 and STK39/SPAK downstream of WNK4, promoting its activity. Phosphorylated in response to IL18. |
| Function | Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1. |
| Protein Name | Solute Carrier Family 12 Member 3Na-Cl CotransporterNccNa-Cl SymporterThiazide-Sensitive Sodium-Chloride Cotransporter |
| Database Links | Reactome: R-HSA-426117Reactome: R-HSA-5619087 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell Membrane |
| Alternative Antibody Names | Anti-Solute Carrier Family 12 Member 3 antibodyAnti-Na-Cl Cotransporter antibodyAnti-Ncc antibodyAnti-Na-Cl Symporter antibodyAnti-Thiazide-Sensitive Sodium-Chloride Cotransporter antibodyAnti-SLC12A3 antibodyAnti-NCC antibodyAnti-TSC antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance