Reagents and instruments for immunology, cell biology and molecular biology.

Arachidonic acid [506-32-1]

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Cat# HY-109590-10mg

Size : 10mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : MedChemExpress

Contact local distributor :

Phone : +1 850 650 7790

Arachidonic acid (Immunocytophyt) is a polyunsaturated omega-6 fatty acid and a major constituent of biomembranes. Arachidonic acid also acts as the substrate for various lipid mediators, such as prostaglandins (PGs). Arachidonic acid improves cognitive response and cardiovascular function.

For research use only. We do not sell to patients.

Arachidonic acid Chemical Structure

CAS No. : 506-32-1

This product is a controlled substance and not for sale in your territory.

Based on 16 publication(s) in Google Scholar

Other Forms of Arachidonic acid:

Arachidonic acid-d₈ In-stock
Arachidonic acid-d₅ In-stock
Arachidonic acid-d₁₁ In-stock
Arachidonic acid sodium salt Get quote
Arachidonic acid (Standard) Get quote

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vivo

Arachidonic acid can be used in animal modeling to construct a rat paw edema model. Arachidonic acid (0.07%, 0.15% or 0.32% in diet; 4 weeks) increases Arachidonic acid content in the paw, but has no effect on arthritis severity and PGE2 content of the paw in a rat arthritis model^[1].

Induction of Paw Edema Model^[2]

Background

Principle: Injecting arachidonic acid into the hind paws of rats can induce rapid and sustained inflammatory responses.

Specific Mmodeling Methods

Rats: Lewis • male •
Administration: 0.5% • s.c. • single dose

Note

Injection method: A single subcutaneous injection of Arachidonic acid in the right hind paw of male Lewis rats (144-241 g) with an injection volume of 0.10 mL (Arachidonic acid is dissolved in 0.2 M carbonate buffer, pH 8.43-8.56 ).

Modeling Indicators

Appearance Monitoring: Significant edema became apparent within 5 minutes, and the reaction reached its peak at 1 hour after injection.

Opposite Product(s): Phenidone (HY-W010144); SK-F86002 (HY-12511)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Lew rats (4-week-old) induced arthritis^[1]
Dosage:	0.07%, 0.15% or 0.32% in diet (w/w)
Administration:	4 weeks
Result:	The Arachidonic acid content of phospholipids in the paw was significantly elevated in a dose-dependent manner.

Clinical Trial

Molecular Weight

304.47

Formula

C₂₀H₃₂O₂

CAS No.

506-32-1

Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(O)=O

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite
Human Gut Microbiota Metabolites

Animals

Microorganisms

mammalian cell

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

Ethanol : 100 mg/mL (328.44 mM; Need ultrasonic)

DMSO : 100 mg/mL (328.44 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2844 mL	16.4220 mL	32.8440 mL
5 mM	0.6569 mL	3.2844 mL	6.5688 mL
10 mM	0.3284 mL	1.6422 mL	3.2844 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.83 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 10 mg/mL (32.84 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.68%

Select Batch:

Data Sheet (270 KB) SDS (0 KB)

COA (237 KB) HNMR (141 KB) RP-HPLC (217 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. Tateishi N, et al. Dietary supplementation with arachidonic acid increases arachidonic acid content in paw, but does not affect arthritis severity or prostaglandin E2 content in rat adjuvant-induced arthritis model. Lipids Health Dis. 2015 Jan 16;14:3. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol / DMSO	1 mM	3.2844 mL	16.4220 mL	32.8440 mL	82.1099 mL
	5 mM	0.6569 mL	3.2844 mL	6.5688 mL	16.4220 mL
	10 mM	0.3284 mL	1.6422 mL	3.2844 mL	8.2110 mL
	15 mM	0.2190 mL	1.0948 mL	2.1896 mL	5.4740 mL
	20 mM	0.1642 mL	0.8211 mL	1.6422 mL	4.1055 mL
	25 mM	0.1314 mL	0.6569 mL	1.3138 mL	3.2844 mL
	30 mM	0.1095 mL	0.5474 mL	1.0948 mL	2.7370 mL
	40 mM	0.0821 mL	0.4105 mL	0.8211 mL	2.0527 mL
	50 mM	0.0657 mL	0.3284 mL	0.6569 mL	1.6422 mL
	60 mM	0.0547 mL	0.2737 mL	0.5474 mL	1.3685 mL
	80 mM	0.0411 mL	0.2053 mL	0.4105 mL	1.0264 mL
	100 mM	0.0328 mL	0.1642 mL	0.3284 mL	0.8211 mL

Arachidonic acid Related Classifications

Metabolic Enzyme/Protease
Endogenous Metabolite

Arachidonic acid [506-32-1]

Cat# HY-109590-10mg

Contact local distributor :

Brand : MedChemExpress

Contact local distributor :

Other Forms of Arachidonic acid:

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Arachidonic acid Related Classifications

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