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> ATG2A Antibody: ATTO 390

ATG2A Antibody: ATTO 390

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Cat# ASM10528-A565-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - ATG2A Antibody: ATTO 390 ASM10528
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG2A Polyclonal Antibody (ASM10528). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (ASM10528) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG2A Antibody (D) Composite.
  • WB - ATG2A Antibody: ATTO 390 ASM10528
    Western blot analysis of Mouse Brain showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (ASM10528). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (ASM10528) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
  • WB - ATG2A Antibody: ATTO 390 ASM10528
    Western blot analysis of Human HeLa cell lysates showing detection of ~212.9kDa ATG2A protein using Rabbit Anti-ATG2A Polyclonal Antibody (ASM10528). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG2A Polyclonal Antibody (ASM10528) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~212.9kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession Q2TAZ0
Other Accession NP_055919.2
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human ATG2A Polyclonal
Target/Specificity Predicted molecular weights at ~213kDa. Other observed bands around ~90-100kDa to be expected.
Other Names ATG2 autophagy related homolog A Antibody, ATG2a_HUMAN Antibody, BC023754 Antibody, KIAA0404 Antibody, 1810013C15Rik Antibody
Immunogen Synthetic peptide from the C-terminal of human ATG2A
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-652 was sufficient for detection of ATG2A on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Preautophagosomal Structure Membrane | Peripheral membrane protein