Reagents and instruments for immunology, cell biology and molecular biology.

Basic Cytotoxicity Test BioAssay™ Kit

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Cat# 474004-125T

Size : 125Tests

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

Contact local distributor :

Phone : +1 850 650 7790

Applications

Shipping Temp

Blue Ice

Storage Temp

4°C/-20°C

Sample Type:

Cell culture

Reagent Name:

7-AAD, CFSE

Target:

Cell-mediated cytotoxicity

Excitation/Emission:

7-AAD–546nm/647nm; CFSE–492nm/520nm

Method of Analysis:

Flow cytometry

Intended Use:

Basic Cytotoxicity Test Assay is a single-tube, dual-color assay for determining cytotoxicity by flow cytometry. The BioAssay™ employs a green fluorescent cellular stain, CFSE, to label target cells and the red live/dead viability dye, 7-AAD, to identify the dead cells present in the cytotoxicity BioAssay™ samples. Analyze your results using a flow cytometer.

Test Principle:

Cytolytic activity is an important process for eliminating intracellular pathogens and cancer cells. Basic Cytotoxicity Assay allows you to assess cytolytic activity in cell culture.

The Basic Cytotoxicity BioAssay™ includes two fluorescent reagents: CFSE and 7-AAD. CFSE, a green fluorescing cellular stain, is utilized to identify the target cell population. The unstained effector cells are added and incubated with the target cells. 7-AAD, a red fluorescing live/dead stain, is then added to stain all necrotic cells red by binding to the DNA of membrane-compromised cells. The target cell and effector cell populations can then be easily distinguished, and flow cytometry easily identifies live and necrotic cells.

Kit Components:

125 tests:
CFSE target cell stain, 1 vial
7-AAD vital stain, 1 vial
10X Assay Buffer, 1x30mL

250 tests:

CFSE target cell stain, 1 vial
7-AAD vital stain, 2 vials
10X Assay Buffer, 1x60mL

Storage and Stability:

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards