Reagents and instruments for immunology, cell biology and molecular biology.

CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I) (HRP)

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Cat# C2398-01N-HRP-100ul

Size : 100ul

Brand : US Biological

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C2398-01N-HRP CD44 (CD 44, CDw44, ECM III, ECMRIII, Epican, Extracellular Matrix Receptor III, GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, Heparan Sulfate Proteoglycan, Hermes Antigen, HUTCH1, Hyaluronate Receptor, Indian Blood Group, Inlu Related p80 Glycoprotein, lhr, Ly 24, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH1, PGP1, Phagocytic Glycoprotein 1, Phagocytic Glycoprotein I) (HRP)

Clone Type

Monoclonal

Host

mouse

Source

human

Isotype

IgG1

Grade

Affinity Purified

Applications

IHC IP

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD44 Iis a a 100kD glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and of the breast. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.

Applications:
Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Use sodium citrate buffer, pH 6, for heat mediated antigen retrieval.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: Bu52|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Specificity: Recognizes the human CD44 cell surface antigen||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes the human CD44 cell surface antigen

References

1. Takahashi, K. et al. (1996) Keratan sulfate modification of CD44 modulates adhesion to hyaluronate. J Biol Chem. 271: 9490-9496. 2. Belitsos, P.C. et al. (1990) Homotypic cell aggregation induced by anti-CD44 (Pgp-1) monoclonal antibodies and related to CD44(Pgp-1) expression. J. Immunol. 144: 1661-1670.