1 vial of lyophilized qSTAR qPCR primer mix (1 nmol each primer, sufficient for 200 reactions). Before use, reconstitute the primer mix in 200 µl dH2O to make a final concentration of 10 µM.
Quality Control
The primer mix has been tested to generate satisfactory qPCR data on ABI 7900HT by using the following PCR program: Stage 1: Activation: 50 °C for 2 min; Stage 2: pre-soak:95 °C for 10 min; Stage 3: Denaturation: 95 °C for 15 sec, Annealing: 60°C for 1 min; Stage 4: Melting curve: 95°C for 15 sec, 60°C for 15 sec, 95°C for 15 sec.
Storage
Store at -20°C.
Stability
The primer mix is stable for one year from date of shipping.
Snippet: The supernatant was stored at −80 °C for subsequent protein extraction. Proprietary qRT-PCR verified primers for human NOS3, VE-cadherin, PECAM-1, thrombomodulin (TM), E-selectin and β-actin were purchased from Origene. ... Cite SaveSaving
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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