CD86 antibody [C86/1146]
Cat# GTX34569-100ug
Size : 100ug
Brand : Genetex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-CD86 antibody [C86/1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34569](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-CD86 antibody [C86/1146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34569")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | C86/1146 |
Isotype | IgG1 |
Application | WB, ICC/IF, IHC-P |
Reactivity | Human, Mouse, Rat |
| Package | 100 μg |
Datasheet File View product citations for antibody GTX34569 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| WB | 1-2μg/ml |
| ICC/IF | 1-2μg/ml |
| IHC-P | 2-4μg/ml for 30 minutes at RT |
| Note : IHC-PStaining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes. | |
Not tested in other applications.
Calculated MW
38 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length CD86 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
B7.2 , B70 , B72 , CD28LG2 , CD86 , CD86 molecule , LAB72 , B7-2
Cellular Localization
Cell membrane
Background
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Database
DATA IMAGES
| | GTX34569 ICC/IF Image ICC/IF analysis of Raji cells using GTX34569 CD86 antibody [C86/1146]. |
| | GTX34569 WB ImageWB analysis of Daudi cell lysate using GTX34569 CD86 antibody [C86/1146]. |
| | GTX34569 IHC-P ImageIHC-P analysis of human melanoma tissue using GTX34569 CD86 antibody [C86/1146]. |
Application Reference
Ko CY et al. Int J Biol Sci 2023; 19 (16) : 5275-5289 Omentin-1 ameliorates the progress of osteoarthritis by promoting IL-4-dependent anti-inflammatory responses and M2 macrophage polarization.
Yunke Liu et al. J Healthc Eng 2022; 2022 : 4441603 Impact of Yiqi Huoxue Decoction on the Relationship between Remodeling of Cardiac Nerves and Macrophages after Myocardial Infarction in Rats.
Application : WB
Fang SY et al. Exp Ther Med 2021; 21 (4) : 369 Distinct phenotypic expression levels of macrophages in neonatal lungs.
Application : WB
Reactivity : Rat
REVIEW
Application Reference
Ko CY et al. Int J Biol Sci 2023; 19 (16):5275-5289 Omentin-1 ameliorates the progress of osteoarthritis by promoting IL-4-dependent anti-inflammatory responses and M2 macrophage polarization.
Yunke Liu et al. J Healthc Eng 2022; 2022:4441603 Impact of Yiqi Huoxue Decoction on the Relationship between Remodeling of Cardiac Nerves and Macrophages after Myocardial Infarction in Rats.
Application : WB
Fang SY et al. Exp Ther Med 2021; 21 (4):369 Distinct phenotypic expression levels of macrophages in neonatal lungs.
Application : WB
Reactivity : Rat
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |
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