This kit contains reagents for labeling antibodies or other proteins with a fluorescent CF® dye succinimidyl ester (CF® dye SE) or biotin succinimidyl ester (biotin SE). Learn more about next-generation CF® dyes with superior brightness, photostability, and water solubility compared to other fluorescent dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

See our full line of Antibody & Protein Labeling Kits.

CF® Dye & Biotin SE Protein Labeling Kits

SE Protein Labeling Kit	Ex/Em	Size	Catalog No.	Dye Features
CF®350	347/448 nm	3 x 1 mg labelings	92210	CF®350 Features
CF®405S	404/431 nm	3 x 1 mg labelings	92211	CF®405S Features
CF®405M	408/452 nm	3 x 1 mg labelings	92212	CF®405M Features
CF®405L	395/545 nm	3 x 1 mg labelings	92228	CF®405L Features
CF®488A	490/515 nm	3 x 1 mg labelings	92213	CF®488A Features
CF®532	527/558 nm	3 x 1 mg labelings	92208	CF®532 Features
CF®543	541/560 nm	3 x 1 mg labelings	92209	CF®543 Features
CF®555	555/565 nm	3 x 1 mg labelings	92214	CF®555 Features
CF®568	562/583 nm	3 x 1 mg labelings	92215	CF®568 Features
CF®594	593/614 nm	3 x 1 mg labelings	92216	CF®594 Features
CF®633	630/650 nm	3 x 1 mg labelings	92217	CF®633 Features
CF®640R	642/662 nm	3 x 1 mg labelings	92225	CF®640R Features
CF®647	650/665 nm	3 x 1 mg labelings	92218	CF®647 Features
CF®660R	663/682 nm	3 x 1 mg labelings	92219	CF®660R Features
CF®660C	667/685 nm	3 x 1 mg labelings	92223	CF®660C Features
CF®680	681/698 nm	3 x 1 mg labelings	92220	CF®680 Features
CF®680R	680/701 nm	3 x 1 mg labelings	92226	CF®680R Features
CF®750	755/777 nm	3 x 1 mg labelings	92221	CF®750 Features
CF®770	770/797 nm	3 x 1 mg labelings	92222	CF®770 Features
Biotin	N/A	3 x 1 mg labelings	92224

CF^® Dye Technical Data

Dye	Abs/Em (nm)	MW (free acid form)	Sephadex® media1	Amax (nm)	Cf A260/Amax	Cf A280/Amax	ε2	Optimal DOL (IgG)
CF®350	355/450	~496	G-25	347	0.13	0.14	18,000	4-6
CF®405S	411/431	~1169	G-25	404	0.19	0.7	33,000	3-5
CF®405M	416/452	~503	G-25	408	0.24	0.13	41,000	4-6
CF®405L	413/547	~1573	G-25	395	N/A	0.5	24,000	8-12
CF®410	404/455	~242	G-25	416	0.15	0.2	46,000	5-7
CF®430	424/497	~429	G-25	426	0.21	0.044	40,000	5-8
CF®440	433/512	~479	G-25	440	0.303	0.139	40,000	5-8
CF®450	448/533	~689	G-25	450	0.205	0.2	40,000	5-8
CF®488A	490/516	~914	G-25	490	0.16	0.1	70,000	7-9
CF®503R	503/532	~1100	G-25	503	0.21	0.09	90,000	4-10
CF®505	505/519	~587	G-25	505	0.22	0.09	90,000	4-8
CF®510	513/537	~562	G-25	513	0.25	0.14	90,000	3-5
CF®514	516/549	~1216	G-25	516	0.14	0.073	105,000	5-8
CF®532	531/552	~685	G-25	527	0.11	0.06	96,000	4-7
CF®543	543/563	~887	G-25	541	0.305	0.095	100,000	4-7
CF®550R	551/577	~686	G-25	551	0.12	0.08	100,000	5-6
CF®555	554/568	~959	G-25	555	0.026	0.08	150,000	4-5, 3-6*
CF®568	562/584	~714	G-25	562	0.17	0.08	100,000	5-6
CF®570	568/592	~2998	G-25	568	0.0998	0.1	150,000	5-6
CF®583	584/606	~3127	G-75	583	0.139	0.223	150,000	5-6
CF®583R	585/609	~773	G-25	585	0.19	0.08	100,000	5-6
CF®594	593/615	~729	G-25	593	0.24	0.08	115,000	4-7
CF®597R	597/619	~800	G-25	597	0.25	0.08	100,000	5-6
CF®620R	620/643	~738	G-25	617	0.28	0.45	115,000	5-6
CF®633	629/650	~821	G-25	630	0.25	0.48	100,000	4-7
CF®640R	642/663	~832	G-50	642	0.23	0.44	105,000	4-7
CF®647	652/668	~985	G-25	650	0.01	0.03	240,000	4-5, 3-6*
CF®660C	667/685	~3024	G-75	667	0.05	0.08	200,000	3-6, 2-3*
CF®660R	662/682	~888	G-25	663	0.2	0.51	100,000	4-7
CF®680	681/698	~3153	G-75	681	0.06	0.09	210,000	3-5, 2-3*
CF®680R	680/701	~912	G-25	680	0.155	0.32	140,000	5-6
CF®700	696/721	~2474	G-75	696	0.055	0.06	240,000	3-6
CF®710	713/735	~860	G-25	712	0.11	0.07	115,000	5-6
CF®725	729/750	~890	G-25	729	0.11	0.07	120,000	5-6
CF®740	742/767	~900	G-25	742	0.132	0.08	105,000	5-6
CF®750	755/779	~2921	G-75	755	0.01	0.03	250,000	3-5, 2-3*
CF®770	770/797	~3091	G-75	770	0.041	0.06	220,000	3-5, 2-3*
CF®790	783/808	~3179	G-75	784	0.104	0.07	210,000	3-5
CF®800	797/817	~3334	G-75	797	0.09	0.08	210,000	3-5
CF®820	822/835	~2711	G-75	822	0.0459	0.07	253,000	3-6
CF®850	852/870	~2787	G-75	852	N/A	0.06	240,000	3-6
CF®870	876/896	~2773	G-75	877	N/A	0.06	240,000	3-6

1. Sephadex® media recommendations are for antibody purification, not nucleic acid.
2. Extinction Coefficient (ε).
*Suitable, but suboptimal DOL.

Sephadex is a registered trademark of Cytiva.

Antibody & Protein Labeling Kits

Product Name	Label Options	Labeling Scale	Labeling Time	Features
Mix-n-Stain™ CF® Dye Antibody Labeling Kits	CF® Dyes	≤5-20 ug IgG 20-50 ug IgG 50-100 ug IgG	~ 15 min.	• Rapid, simple labeling • No purification • Tolerates BSA & other additives • Dyes for super-resolution, spectral flow, & NIR detection
Mix-n-Stain™ FITC Antibody Labeling Kits	FITC
Mix-n-Stain™ Cyanine Dye Antibody Labeling Kits	Cyanine 555 Cyanine 647
Mix-n-Stain™ Biotin Antibody Labeling Kits	Biotin
Mix-n-Stain™ Digoxigenin Antibody Labeling Kits	Digoxygenin (DIG)
Mix-n-Stain™ DNP Antibody Labeling Kits	DNP
Mix-n-Stain™ Maxi Antibody Labeling Kits	CF® Dyes Cyanine Dyes	1 mg IgG	~ 30 min.
Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits	CF® Dyes FITC	25 ug IgM 100 ug IgM	15-30 min.	• Rapid, simple labeling • No purification
Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits	CF® Dyes	50 ug IgG	~ 30 min.	• Optimized to provide low 1-2.5 DOL • No purification
Mix-n-Stain™ Nanobody Labeling Kits	CF® Dyes	5-20 ug Nanobody® 20-50 ug Nanobody®	~ 30 min.	• Optimized for Nanobodies® • Tolerates BSA & other additives
Mix-n-Stain™ Nanobody Thiol Labeling Kits	CF® Dyes		~ 2 hours	• Optimized for Nanobodies® with single exposed cysteine residue
Mix-n-Stain™ Small Ligand Labeling Kits	CF® Dyes	0.1 umol small ligand	~ 30 min.	• Label SNAP®, CLIP™, HaloTag® & other small ligands • Dye options for surface or intracellular targets
VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging	Near-IR CF® Dyes	3 x 1 mg IgG	~ 2 hours	• Dyes, buffers, & spin vials for labeling + purification • Sterile syringes, filters and storage vials for in vivo use
CF® Dye & Biotin SE Protein Labeling Kits	CF® Dyes Biotin	3 x 1 mg protein	~ 2 hours	• Dyes, buffers, & spin vials for labeling + purification