In the presence of ATP, magnesium ions and oxygen, Luciferase (also known as Firefly Luciferase) can catalyze firefly luciferin to Oxyluciferin, which generate light signals during the oxidation of luciferin. In a certain range, the fluorescence intensity is proportional to the concentration of ATP.
Images & Validation
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Key Properties
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Assay Type
Detection
Storage & Handling
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Storage
Stored at -20°C for 12 months, protected from light