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> Dog S100 calcium binding protein B (S100B) ELISA kit

Dog S100 calcium binding protein B (S100B) ELISA kit

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Cat# CSB-EL020643DO-24T

Size : 24T

Brand : Cusabio

SDS

CSB-EL020643DO
Size 96T,5×96T,10×96T

Product Details

Target Name
S100 calcium binding protein B (S100B)
Abbreviation
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
39 pg/mL-2500 pg/mL
Sensitivity
9.8 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig S100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 85    
Range % 80-91    
1:2 Average % 93    
Range % 87-98    
1:4 Average % 102    
Range % 98-106    
1:8 Average % 98    
Range % 94-103    
Recovery
The recovery of pig S100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 89-102    
EDTA plasma (n=4) 89 84-95    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2500 1.955 2.021 1.988 1.816    
1250 1.474 1.522 1.498 1.326    
625 0.911 0.987 0.949 0.777    
312.5 0.526 0.536 0.531 0.359    
156.2 0.365 0.345 0.355 0.183    
78.1 0.293 0.312 0.303 0.131    
39 0.221 0.226 0.224 0.052    
0 0.175 0.169 0.172 　    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months

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