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> FOLR4/Juno Protein, Mouse, Recombinant (His)

FOLR4/Juno Protein, Mouse, Recombinant (His)

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Cat# NB-64-54869-50ug

Size : 50ug

Brand : Neo Biotech


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FOLR4/Juno Protein, Mouse, Recombinant (His)

(Synonyms: Juno, Izumo1r, FR-δ, FR-delta, FR δ, FR delta, FOLR4, LOC390243, Folbp3, Folate receptor 4) Copy Product Info

Synonyms: Juno, Izumo1r, FR-δ, FR-delta, FR δ, FR delta, FOLR4, LOC390243, Folbp3, Folate receptor 4

Catalog No. TMPK-01171 Copy Product Info
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor. FOLR4/Juno Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 24.7 kDa and the accession number is Q9EQF4-1.
Other Forms of FOLR4/Juno Protein, Mouse, Recombinant (His):
FOLR4/Juno Protein, Human, Recombinant (His)TMPK-00585
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For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor. FOLR4/Juno Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 24.7 kDa and the accession number is Q9EQF4-1.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberQ9EQF4-1
ConstructionGly20-Gly222
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsJuno, Izumo1r, FR-δ, FR-delta, FR δ, FR delta, FOLR4, LOC390243, Folbp3, Folate receptor 4
Research Background
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.
Chemical Properties
Molecular Weight24.7 kDa (predicted). Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

FOLR4, LOC-390243FOLR4, LOC 390243
Related Tags: FOLR4/Juno Protein, Mouse, Recombinant (His) chemical structure | FOLR4/Juno Protein, Mouse, Recombinant (His) molecular weight