ASM10321-STR-100 | GABA-A Receptor Alpha 4 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34 (ASM10321). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody (ASM10321) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha4 Antibody (D) Composite.
Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (ASM10321). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (ASM10321) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immunoelectronmicroscopy EIA=Enzyme Immunoassay	WB, IHC, ICC/IF
Primary Accession	P28471
Other Accession	NP_542154.3
Host	Mouse
Isotype	IgG1
Reactivity	Human, Mouse, Rat
Clonality	Monoclonal
Description	Mouse Anti-Rat GABA-A Receptor Alpha4 Monoclonal IgG1
Target/Specificity	Detects ~60kDa. Does not cross-react with other GABA-A-Receptor Alpha subunits.
Other Names	GABA A receptor subunit alpha 4 Antibody, GABRA4 Antibody, Gamma aminobutyric acid A receptor Alpha 4 Antibody
Clone Names	S398A-34
Immunogen	Fusion protein amino acids 410-450 (Cytoplasmic C-terminus) of rat GABA-A-Receptor Alpha 4
Purification	Protein G Purified
Storage	-20ºC
Storage Buffer	PBS pH7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:100 dilution of SMC-489 was sufficient for detection of GABA-A R, Alpha4 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization	Cell Junction \| Synapse \| Postsynaptic Cell Membrane \| Cell Membrane

GABA-A Receptor Alpha 4 Antibody