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> GluN2B/NR2B Antibody

GluN2B/NR2B Antibody

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Cat# ASM10209-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • IHC - GluN2B/NR2B Antibody ASM10209
    Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-20 (ASM10209). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (ASM10209) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive (dull) suprabasal staining in the epidermis and some hair follicle localization.
  • WB - GluN2B/NR2B Antibody ASM10209
    Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-20 (ASM10209). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody (ASM10209) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC/IF, IP, AM
Primary Accession Q00960
Other Accession NP_036706.1
Host Mouse
Isotype IgG2a
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GluN2B/NR2B Monoclonal IgG2a
Target/Specificity Detects ~166kDa. No cross-reactivity against NR2A.
Other Names NMDAR2B Antibody, hNR3 Antibody, MGC142180 Antibody, glutamate receptor subunit epsilon-2 Antibody, NR3 Antibody, GluN2B Antibody, hNR3 Antibody, MRD6 Antibody, NMDAR2B Antibody, NR2B Antibody
Clone Names S59-20
Immunogen Fusion protein amino acids 20-271 (N-terminus) of rat NR2B
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-333 was sufficient for detection of NR2B/GluN2B in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Cell Junction | Synapse | Postsynaptic Cell Membrane