Reagents and instruments for immunology, cell biology and molecular biology.
 
> Goat Rabbit IgM (Fc specific), conjugated with Horseradish peroxidase Antibody

Goat Rabbit IgM (Fc specific), conjugated with Horseradish peroxidase Antibody

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Cat# orb21470-1ml

Size : 1ml

Brand : Biorbyt


Goat Rabbit IgM (Fc specific), conjugated with Horseradish peroxidase Antibody

SKU: orb21470
This product can be custom made

Description

Horseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to Rabbit IgM, Fc specific. In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demon- strate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of rabbit origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in rabbit serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,000 and 1:10,000.

Research Area

Cell Biology

Images & Validation

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Goat Rabbit IgM (Fc specific), conjugated with Horseradish peroxidase Antibody
ReactivityRabbit

Key Properties

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Antibody TypeSecondary Antibody
HostGoat
ClonalityPolyclonal
ConjugationHRP

Storage & Handling

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StorageThe lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
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Protocol Information