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> GRP78 Antibody

GRP78 Antibody

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Cat# ASM10155-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - GRP78 Antibody ASM10155
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 (ASM10155). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (ASM10155) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
  • WB - GRP78 Antibody ASM10155
    Western Blot analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 (ASM10155). Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody (ASM10155) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF, E
Primary Accession P06761
Other Accession NP_037215.1
Host Mouse
Isotype IgG1 Kappa
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GRP78 Monoclonal IgG1 Kappa
Target/Specificity Detects ~78kDa.
Other Names 78 kDa glucose regulated protein Antibody, 78 kDa glucose-regulated protein Antibody, AL022860 Antibody, AU019543 Antibody, BIP Antibody, D2Wsu141e Antibody, D2Wsu17e Antibody, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 Antibody, Endoplasmic reticulum lumenal Antibody, Ca2+ binding protein grp78 Antibody, FLJ26106 Antibody, Glucose Regulated Protein 78kDa Antibody, GRP 78 Antibody, GRP-78 Antibody, GRP78_HUMAN Antibody, Heat shock 70 kDa protein 5 Antibody, Heat Shock 70kDa Protein 5 Antibody, HSCe70 Antibody, HSPA 5 Antibody, HSPA5 Antibody, Immunoglobulin Heavy Chain Binding Protein Antibody, Immunoglobulin heavy chain-binding protein Antibody, mBiP Antibody, MIF2 Antibody, Sez7 Antibody
Clone Names 3C5-1A4
Immunogen Full-length recombinant rat GRP78
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-210 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum | Endoplasmic Reticulum Lumen | Melanosome