GP31 | Guinea Pig Polyclonal Anti-Perilipin 5 (C-terminus) Clinisciences

anti-Perilipin 5 (C-terminus) guinea pig polyclonal, serum

Key Features

Stabilized antiserum
Guinea pig polyclonal
Suitable for ICC/IF and IHC
Reacts with bovine, human, mouse and rat

Product description

Quantity	100 µl
Antibody Type	Polyclonal
Host	Guinea pig
Conjugate	Unconjugated
Application	ICC/IF, IHC
Purification	Stabilized antiserum
Reactivity	Bovine, Human, Mouse, Rat
Storage	Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles
Intended use	Research use only
Immunogen	Synthetic peptide (C-terminal aa 451-463 of human MLDP; CPVKHTLMPELDF)
Formulation	Contains 0.09% sodium azide
UniprotID	A6QLL0 (Bovine), Q00G26 (Human), Q8BVZ1 (Mouse), M0R7Z9 (Rat)
Synonym	Perilipin-5, Lipid storage droplet protein 5, PLIN5, LSDP5, OXPAT, PAT-1
Note	Centrifuge prior to opening

Applications

Tested applications	Tested dilutions
Immunocytochemistry (ICC)/ Immunofluorescence (IF)	Assay dependent
Immunohistochemistry (IHC) - frozen	1:100-1:300
Immunohistochemistry (IHC) - paraffin	1:100-1:300 (microwave treatment recommended)

Background

Polypeptide reacting: MLDP = myocardial lipid droplet protein (also described as OXPAT/PAT-1 and perilipin 5/PLIN5), MW 50,844 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE)
MLDP/PLIN5 pertains to the PLIN/PAT-family proteins, covering the surface of cytoplasmic lipid droplets and sharing a homologuous domain called PAT.
No cross-reactivity with additional PLIN/PAT proteins (including adipophilin/ADRP/PLIN2, perilipin/PLIN1, and TIP47/PLIN3) which are expressed in differentiation-related stages of lipid metabolism.
Immunolocalization: MLDP/ PLIN5 is positively detected in heart and skeletal muscle.

References/Publications (48)

Publication

Species

Application

Publication Cinato, M. et al. Cardiac Plin5 interacts with SERCA2 and promotes calcium handling and cardiomyocyte contractility. Life Sci Alliance 6, (2023).

Species mouse

Application WB

Publication Fachada, V. et al. Effects of Long-Term Physical Activity and BCAA Availability on the Subcellular Associations between Intramyocellular Lipids, Perilipins and PGC-1α. Int J Mol Sci 24, (2023).

Application IHC

Publication Krizanac, M., Mass Sanchez, P. B., Schröder, S. K., Weiskirchen, R. & Asimakopoulos, A. Lipid-Independent Regulation of PLIN5 via IL-6 through the JAK/STAT3 Axis in Hep3B Cells. Int J Mol Sci 24, (2023).

Application ICC-IF

Publication Fachada, V. et al. Enlarged PLIN5-uncoated lipid droplets in inner regions of skeletal muscle type II fibers associate with type 2 diabetes. Acta Histochem 124, (2022).

Species human

Application IHC

Publication Schelbert, S. et al. Lipid Droplet-Associated Proteins Perilipin 1 and 2: Molecular Markers of Steatosis and Microvesicular Steatotic Foci in Chronic Hepatitis C. Int J Mol Sci 23, (2022).

Species human

Application IHC

Publication Eynaudi, A. et al. Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2. Front Nutr 8, (2021).

Species human

Application WB

Publication Mareninova, O. A. et al. Dysregulation of mannose-6-phosphate–dependent cholesterol homeostasis in acinar cells mediates pancreatitis. Journal of Clinical Investigation 131, (2021).

Species human

Publication Keenan, S. N. et al. Perilipin 5 S155 phosphorylation by PKA is required for the control of hepatic lipid metabolism and glycemic control. J Lipid Res 62, (2021).

Species mouse

Application WB

Publication Daemen, S. et al. Postexercise changes in myocellular lipid droplet characteristics of young lean individuals are affected by circulatory nonesterified fatty acids. Am J Physiol Endocrinol Metab 321, E453–E463 (2021).

Species human

Application IHC

Publication van Polanen, N. et al. Resveratrol-induced remodelling of myocellular lipid stores: A study in metabolically compromised humans. Physiol Rep 9, (2021).

Species human

Application IHC

Publication Talbott, H. et al. Formation and characterization of lipid droplets of the bovine corpus luteum. Sci.Rep. 10, 11287 (2020)

Species bovine

Application WB

Publication Whytock, K. L. et al. A 7-day high-fat, high-calorie diet induces fibre-specific increases in intramuscular triglyceride and perilipin protein expression in human skeletal muscle. Journal of Physiology 598, 1151–1167 (2020).

Species human

Application IHC (frozen)-IF

Publication Shaw, C. S. et al. Impact of exercise training status on the fiber type-specific abundance of proteins regulating intramuscular lipid metabolism. J Appl Physiol 128, 379–389 (2020).

Species human

Application WB

Publication Zhu, Y., Ren, C., Zhang, M. & Zhong, Y. Perilipin 5 Reduces Oxidative Damage Associated With Lipotoxicity by Activating the PI3K/ERK-Mediated Nrf2-ARE Signaling Pathway in INS-1 Pancreatic β-Cells. Front Endocrinol (Lausanne) 11, 166 (2020).

Species rat

Application WB

Publication Jevons, E. F. P., et al. Skeletal muscle lipid droplets are resynthesized before being coated with perilipin proteins following prolonged exercise in elite male triathletes. Am J Physiol Endocrinol Metab 318, E357–E370 (2020).

Species human

Application IHC(frozen)-IF

Publication Irungbam, K. et al. Cannabinoid receptor 1 knockout alleviates hepatic steatosis by downregulating perilipin 2. Lab.Invest. 100, 454-465 (2020)

Species mouse

Application WB

Publication Kang, S. W. S., Cogger, V. C., Le Couteur, D. G. & Fu, D. Multiple cellular pathways regulate lipid droplet homeostasis for the establishment of polarity in collagen sandwich-cultured hepatocytes. Am J Physiol Cell Physiol 317, C942–C952 (2019).

Species rat

Application WB

Publication Straub, B. K. et al. Perilipin 1 Expression Differentiates Liposarcoma from Other Types of Soft Tissue Sarcoma. American Journal of Pathology 189, 1547–1558 (2019).

Species human

Application IHC, WB

Publication Zhu, Y. et al. Perilipin5 protects against lipotoxicity and alleviates endoplasmic reticulum stress in pancreatic β-cells. Nutr.Metab.(Lond). 16, 50 (2019)

Species mouse,rat

Application WB

Publication Lee, M. et al. Rosiglitazone remodels the lipid droplet and britens human visceral and subcutaneous adipocytes ex vivo. J.Lipid.Res. 60, 856-868 (2019)

Species human

Application WB

Publication Mardani, I. et al. Plin2-deficiency reduces lipophagy and results in increased lipid accumulation in the heart. Sci.Rep. 9, 6909 (2019)

Species mouse

Application WB,ICC-IF

Publication Lian, J. et al. Ces1d deficiency protects against high-sucrose diet-induced hepatic triacylglycerol accumulation. J.Lipid.Res. 60, 880-891 (2019)

Species mouse

Application WB

Publication Gemmink, A. et al. Super-resolution microscopy localizes perilipin 5 at lipid droplet-mitochondria interaction sites and at lipid droplets juxtaposing to perilipin 2. Biochim Biophys Acta Mol Cell Biol Lipids 1863, 1423–1432 (2018).

Species human

Application IHC(frozen)-IF, IHC

Publication Daemen, S. et al. Distinct lipid droplet characteristics and distribution unmask the apparent contradiction of the athlete's paradox. Mol.Metab. 17, 71-81 (2018).

Species human

Application WB

Publication Whytock, K. et al. Hormone-sensitive lipase preferentially redistributes to lipid droplets associated with perilipin-5 in human skeletal muscle during moderate-intensity exercise. J.Physiol. 596, 2077-2090 (2018)

Species human

Application IHC-IF (frozen)

Publication Gemmink, A. et al. Dissociation of intramyocellular lipid storage and insulin resistance in trained athletes and type 2 diabetes patients; involvement of perilipin 5? J. Physiol. (2017).

Species human

Application WB

Publication Gemmink, A. et al. Lipid droplet dynamics and insulin sensitivity upon a 5-day high-fat diet in Caucasians and South Asians. Sci. Rep. 7, 1–9 (2017).

Species human

Application WB

Publication Laurens, C. et al. Perilipin 5 fine-tunes lipid oxidation to metabolic demand and protects against lipotoxicity in skeletal muscle. Sci. Rep. 6, 1–12 (2016).

Species human, mouse

Application WB

Publication Barquissau, V. et al. White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways. Mol. Metab. 5, 352-365 (2016).

Species human

Application WB

Publication Gemmink, A. et al. Decoration of intramyocellular lipid droplets with PLIN5 modulates fasting-induced insulin resistance and lipotoxicity in humans. Diabetologia 59, 1040-8 (2016).

Species human

Application WB,IHC (frozen)

Publication Gallardo-Montejano, V. I. et al. Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1a/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat. Commun. 7, (2016).

Species mouse

Application PLA,WB,ICC-IF,IP

Publication Pourteymour, S. et al. Perilipin 4 in human skeletal muscle: localization and effect of physical activity. Physiol. Rep. 3, (2015).

Species human

Application IHC (paraffin)

Publication Trevino, M. B. et al. Perilipin 5 regulates islet lipid metabolism and insulin secretion in a cAMP-dependent manner: Implication of its role in the postprandial insulin secretion. Diabetes 64, 1299-1310 (2015).

Species human,mouse

Application WB,ICC-IF

Publication Billecke, N. et al. Perilipin 5 mediated lipid droplet remodelling revealed by coherent Raman imaging. Integr. Biol. (Camb). 7, 467-76 (2015).

Species rat

Application IHC (frozen)

Publication Kuramoto, K. et al. Deficiency of a Lipid Droplet Protein, Perilipin 5, Suppresses Myocardial Lipid Accumulation, Thereby Preventing Type 1 Diabetes- Induced Heart Malfunction. Mol. Cell. Biol. -34, 2721-2731 (2014).

Species mouse

Application WB

Publication Mason, R. R., Meex, R. C. R., Russell, A. P., Canny, B. J. & Watt, M. J. Cellular Localization and Associations of the Major Lipolytic Proteins in Human Skeletal Muscle at Rest and during Exercise. PLoS One 9, (2014).

Species human

Application IHC (frozen)

Publication Mason, R. R. et al. PLIN5 deletion remodels intracellular lipid composition and causes insulin resistance in muscle. Mol. Metab. 3, 652-663 (2014).

Species mouse

Application IHC (frozen)

Publication Ramos, S. V et al. Higher PLIN5 but not PLIN3 content in isolated skeletal muscle mitochondria following acute in vivo contraction in rat hindlimb. Physiol. Reports 2, (2014).

Species rat

Application WB,Co-IP

Publication MacPherson, R. E. K., Ramos, S. V, Vandenboom, R., Roy, B. D. & Peters, S. J. Skeletal muscle PLIN proteins, ATGL and CGI-58, interactions at rest and following stimulated contraction. Am. J. Physiol. Regul. Integr. Comp. Physiol. 304, R644-50 (2013).

Species rat

Application WB,Co-IP

Publication Heid, H. et al. Lipid droplets, perilipins and cytokeratins--unravelled liaisons in epithelium-derived cells. PLoS One 8, (2013).

Species human

Application WB,ICC-IF

Publication Dahlhoff, M. et al. PLIN2, the major perilipin regulated during sebocyte differentiation, controls sebaceous lipid accumulation in vitro and sebaceous gland size in vivo NIH Public Access. Biochim. Biophys. Acta 1830, 4642-4649 (2013).

Species human

Application IHC (paraffin)

Publication Macpherson, R. E. K., Vandenboom, R., Roy, B. D. & Peters, S. J. Skeletal muscle PLIN3 and PLIN5 are serine phosphorylated at rest and following lipolysis during adrenergic or contractile stimulation. Physioligal Reports 1, (2013).

Species rat

Application WB

Publication Timmers, S. et al. Augmenting muscle diacylglycerol and triacylglycerol content by blocking fatty acid oxidation does not impede insulin sensitivity. Proc. Natl. Acad. Sci. USA 109, 11711-11716 (2012).

Species mouse

Application WB

Publication Bosma, M. et al. The lipid droplet coat protein perilipin 5 also localizes to muscle mitochondria. Histochem Cell Biol 137, 205-216 (2012).

Species human,rat

Application WB,ICC-IF,IEM

Publication Macpherson, R. E. K. et al. Subcellular localization of skeletal muscle lipid droplets and PLIN family proteins OXPAT and ADRP at rest and following contraction in rat soleus muscle. Am. J. Physiol. - Regul. Integr. Comp. Physiol. -(2011).

Species rat

Application IHC (frozen)

Publication Timmers, S. et al. Paradoxical Increase in TAG and DAG Content Parallel the Insulin Sensitizing Effect of Unilateral DGAT1 Overexpression in Rat Skeletal Muscle. PLoS One 6, (2011).

Species rat

Application WB

Publication Wang, H. et al. Unique regulation of adipose triglyceride lipase (ATGL) by perilipin 5, a lipid droplet-associated protein. J. Biol. Chem. 286, 15707-15715 (2011).

Species human

Application WB

Publication Yamaguchi, T., Matsushita, S., Motojima, K., Hirose, F. & Osumi, T. MLDP, a novel PAT family protein localized to lipid droplets and enriched in the heart, is regulated by peroxisome proliferator-activated receptor alpha. J. Biol. Chem. 281, 14232-40 (20

Species mouse

Application WB,ICC-IF

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Datasheet

MSDS

FAQs

Most of our purified mouse antibodies contain 0.5% BSA as stabilizer. If BSA was added to the antibody solution, it is stated in the datasheet.
The supernatant format contains FCS proteins from cell culture medium supplemented with FCS.
The serum antibodies contain other proteins present in serum.

Lyophilized antibodies can be stored at 2-8°C until expiration.
Most of our liquid antibodies and reconstituted lyophilized antibodies may be stored for short term storage (up to 3 month) at 2-8°C. For long term storage we recommend to store the antibody at -20°C in aliquots. Please avoid freeze and thaw cycles.
Most of our conjugated antibodies should be stored at 2-8°C.
The individual storage conditions are mentioned on the datasheet.

The expiration date of our antibodies is indicated on the product label.

Methanol/ acetone fixation: Immerse slide in precooled (-20°C) methanol for 5 min, immerse in precooled (-20°C) acetone for 30-60 sec, let specimen air dry before antibody incubation.
Methanol/ acetone fixation plus detergent permeabilization: After methanol/ acetone fixation and air-drying dip slide either in a solution containing 0.1-0.2% Triton X-100 in PBS or in 0.1% saponin in PBS for 1-5 min at room temperature (enhances accessibility of many cytoskeletal antigens).

Air-drying of the section.
Block with the serum of the species in which the secondary antibody was raised for 30 min.
Incubation with 1st antibody 1 h at RT in moist chamber.
Wash 3x with PBS.
Incubation with appropriate fluorescent secondary antibody, 30-60 min at RT.
Wash 3x with PBS.
Immerse shortly into ethanol.
Let air dry.
Cover with mounting medium.

Most of our antibodies contain 0.09% sodium azide as preservative. If a preservative is added, it is mentioned in the datasheet.

The optimal antibody dilution for your specific protocol and application needs to be titrated in your lab with your equipment and sample. The optimal dilution may vary between protocols and samples. A good dilution for starting the titration is the dilution mentioned in the datasheet. If the sample needs a specific treatment (eg. Antigen retrieval for IHC on FFPE sections) this should also be mentioned on the antibody datasheet.

PROGEN antibodies are shipped at ambient temperature. The antibodies are stable at ambient temperature for the shipment period. Please store the antibodies as indicated in the datasheet upon arrival.