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> HLA Class 2 Antigen DQ3 (X-Reactive)

HLA Class 2 Antigen DQ3 (X-Reactive)

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Cat# MBS603511-0,1mL

Size : 0,1mL

Brand : MyBiosource

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anti-HLA-A antibody :: Mouse anti-Human HLA Class 2 Antigen DQ3 Monoclonal Antibody

anti-HLA-A antibody
Product Name

HLA Class 2 Antigen DQ3 (HLA-A), Monoclonal Antibody

Full Product Name

HLA Class 2 Antigen DQ3 (X-Reactive)

Product Synonym Names
Anti -HLA Class 2 Antigen DQ3 (X-Reactive)
Product Gene Name

anti-HLA-A antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
MBS603511 COA
COA PDF
MBS603511 Testing Data
Testing Data PDF
MBS603511 QC Report
QC Report PDF
MBS603511 MSDS
MSDS PDF
MBS603511 Technical Datasheet
Technical Datasheet PDF
MBS603511 Protocol
Protocol PDF
Molecular Weight
40,909 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (29910247..29913661). Location: 6p21.3
OMIM
142800
3D Structure
ModBase 3D Structure for P01891
Clonality
Monoclonal
Isotype
IgM
Clone Number
4i145
Host
Mouse
Species Reactivity
Human
Specificity
Reacts to HLA Class 2 Antigen-DQ3. Additional crossreactivity occurs with related proteins.. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Purity/Purification
Ascites
Ascites
Form/Format
Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution.
Immunogen
HLA Class 2 Antigen-DQ3.
Cytotoxicity
Expected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Please contact MyBioSource for bulk requirements and licensing requests relating to production applications.
Other Notes
Small volumes of anti-HLA-A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-HLA-A antibody
Antibodies; Abs to HLA
Application Notes for anti-HLA-A antibody
Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies.
Dilution: Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
Sample Manual Insert of MBS603511.
NCBI/Uniprot data below describe general gene information for HLA-A. It may not necessarily be applicable to this product.
NCBI GI #
1561526
NCBI GeneID
3105
NCBI Accession #
BAA11936.1
NCBI Official Synonym Symbols
HLAA
  
NCBI Protein Information
HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain
UniProt Protein Name
HLA class I histocompatibility antigen, A-68 alpha chain
UniProt Synonym Protein Names
Aw-68; HLA class I histocompatibility antigen, A-28 alpha chain; MHC class I antigen A*68
Protein Family
HLA class I histocompatibility antigen
UniProt Gene Name
HLA-A  
UniProt Synonym Gene Names
HLAA  
UniProt Entry Name
1A68_HUMAN
NCBI Summary for HLA-A
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
UniProt Comments for HLA-A
HLAA: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family.

Protein type: Immunoglobulin superfamily; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; Golgi apparatus; phagocytic vesicle membrane; cell surface; membrane; early endosome membrane; integral to plasma membrane; endoplasmic reticulum; plasma membrane; MHC class I protein complex; Golgi medial cisterna

Molecular Function: protein binding; peptide antigen binding; beta-2-microglobulin binding; T cell receptor binding; TAP binding; receptor binding

Biological Process: regulation of immune response; viral reproduction; detection of bacterium; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protection from natural killer cell mediated cytotoxicity; regulation of defense response to virus by virus; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent; positive regulation of interferon-gamma production; antigen processing and presentation of peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I; immune response

Disease: Severe Cutaneous Adverse Reaction, Susceptibility To
Research Articles on HLA-A
1. The most frequent haplotypes that may be associated with susceptibility to American cutaneous leishmaniasis were A*02 B*44 DRB1*07 (P = 0.0236) and A*24 B*35 DRB1*01 (P = 0.0236).