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> HO-1 (Rat) Antibody

HO-1 (Rat) Antibody

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Cat# ASM10162-STR-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - HO-1 (Rat) Antibody ASM10162
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1 (Rat) Monoclonal Antibody, Clone 6B8-2F2 (ASM10162). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HO-1 (Rat) Monoclonal Antibody (ASM10162) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.
  • WB - HO-1 (Rat) Antibody ASM10162
    Western Blot analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2 (ASM10162). Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody (ASM10162) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P06762
Other Accession NP_036712.1
Host Mouse
Isotype IgG1 Kappa
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat HO-1 Monoclonal IgG1 Kappa
Target/Specificity Detects ~32kDa. Does not cross-react with HO-2.
Other Names HSP32 Antibody, HMOX1 Antibody, Heme oxygenase 1 Antibody, HO Antibody, HO1 Antibody
Clone Names 6B8-2F2
Immunogen His-tagged Rat HO-1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-234 was sufficient for detection of HO-1 in 10 µg of rat kidney lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Microsome | Endoplasmic Reticulum