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> HSP60 Antibody

HSP60 Antibody

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Cat# ASM10360-P594-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - HSP60 Antibody ASM10360
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody (ASM10360). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody (ASM10360) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
  • WB - HSP60 Antibody ASM10360
    Western blot analysis of Human, Dog, Mouse SKBR3, MDCK, and MEF cell line lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (ASM10360). Lane 1: Recom. Human Hsp60 (100ng), Lane2, 3 and 4: SKBR3 lysate (human), MDCK lysate (dog) and MEF lysate (mouse) (al at 7.5ug). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (ASM10360) at 1:1000.
  • ICC/IF - HSP60 Antibody ASM10360
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody (ASM10360). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody (ASM10360) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Mitochondrion matrix. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IP
Primary Accession P10809
Other Accession NP_002147.2
Host Rabbit
Reactivity Human, Mouse, Rat, Rabbit, Chicken, Bovine, Dog
Clonality Polyclonal
Description Rabbit Anti-Human HSP60 Polyclonal
Target/Specificity Detects ~60kDa.
Other Names CPN60 Antibody, GROEL Antibody, HLD4 Antibody, HSP 60 Antibody, HSP65 Antibody, HSPD1 Antibody, HuCHA60 Antibody, SPG 13 Antibody
Immunogen Human HSP60 produced through recombinant DNA methods in E.coli
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-105 was sufficient for detection of HSP60 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
Cellular Localization Mitochondrion | Mitochondrion Matrix