RayBio® G-Series Human Angiogenesis Antibody Array 1 Kit. Detects 20 Human Angiogenic Factors. Suitable for all liquid sample types.
Human Angiogenesis Array G1
Human Angiogenesis Array G1
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Angiogenesis, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| EGF
| ENA-78 (CXCL5)
| bFGF
| GRO alpha/beta/gamma
|
IFN-gamma
| IGF-1
| IL-6
| IL-8 (CXCL8)
| Leptin
|
MCP-1 (CCL2)
| PDGF-BB
| PLGF
| RANTES (CCL5)
| TGF beta 1
|
TIMP-1
| TIMP-2
| Thrombopoietin (TPO)
| VEGF-A
| VEGF-D
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
- Human Angiogenesis Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-ANG-G1-4.gal)
GAL (GAL-AAH-ANG-G1-8.gal)
Excel (AAH-ANG-G1_Analysis_Tool.xls)
citations
Microvesicles of pregnant women receiving low molecular weight heparin improve trophoblast function
- Species:
- Human
- Sample Type:
- Conditioned Media (Microvesicle specimens)
- Year:
- 2016
- Journal Name:
- Thrombosis Research
- PubMed ID:
- 26639203
In vitro and in vivo anti-angiogenic activity of girinimbine isolated from Murraya koenigii
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Umbilical Veiwn endothelial Cells, HUVEC)
- Year:
- 2015
- Journal Name:
- Drug design, development and therapy
Angiogenin secretion from hepatoma cells activates hepatic stellate cells to amplify a self-sustained cycle promoting liver cancer
- Species:
- Human
- Sample Type:
- Conditioned Media (HepG2, Hep3B, and LX2 cells)
- Year:
- 2015
- Journal Name:
- Scientific reports
- PubMed ID:
- 25604905
Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels
- Species:
- Human
- Sample Type:
- Hydrogel (Bone Marrow Derived Meschencymal Stem Cells embedded in a hydrogel media)
- Year:
- 2014
- Journal Name:
- Acta Biomaterialia
- PubMed ID:
- 24468583
Late outgrowth endothelial cells resemble mature endothelial cells and are not derived from bone marrow.
- Species:
- Human
- Sample Type:
- Conditioned Media (EOC and HUVEC cell cultures)
- Year:
- 2013
- Journal Name:
- Stem Cells
- PubMed ID:
- 23165527
Ionizing radiation and glioblastoma exosomes: implications in tumor biology and cell migration
- Species:
- Human
- Sample Type:
- Cell Lysate (Exosome lysate)
- Year:
- 2013
- Journal Name:
- Translational Oncology
- PubMed ID:
- 24466366
Lysophosphatidic acid enhances stromal cell-directed angiogenesis.
- Species:
- Human
- Sample Type:
- Conditioned Media (Human adipose-derived stromal cells)
- Year:
- 2013
- Journal Name:
- Plos One
- PubMed ID:
- 24312635
Cardioprotection by placenta-derived stromal cells in a murine myocardial infarction model.
- Species:
- Human
- Sample Type:
- Conditioned Media (PLX-PAD cells under hypoxia)
- Year:
- 2013
- Journal Name:
- Journal of Surgical Research
- PubMed ID:
- 23830369
Bronchial smooth muscle cells of asthmatics promote angiogenesis through elevated secretion of CXC-chemokines (ENA-78, GRO-, and IL-8).
- Species:
- Human
- Sample Type:
- Conditioned Media ( bronchial smooth muscle cells)
- Year:
- 2013
- PubMed ID:
- 24339939
MicroRNA-214 inhibits angiogenesis by targeting Quaking and reducing angiogenic growth factor release
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Microvascular Endothelial Cells Culture Sup)
- Year:
- 2012
- Journal Name:
- Cardiovascular Research
- PubMed ID:
- 22227154