Human Cathelicidin antimicrobial peptide(CAMP) ELISA kit

Product Details

Citations

Customer Reviews and Q&A

Brand : Cusabio

Cat# CSB-EL004476HU-96T

Reagents and instruments for immunology, cell biology and molecular biology.

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Size : 96T

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CSB-EL004476HU

Size	96T,5×96T,10×96T

Associated names

CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37,

Abbreviation

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.45 ng/mL-30 ng/mL

Sensitivity

0.11 ng/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
Range %	80-92
1:200	Average %	96
Range %	91-105
1:400	Average %	99
Range %	92-110
1:800	Average %	95
Range %	86-98

Recovery

The recovery of human CAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	89-98
EDTA plasma (n=4)	95	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
30	2.713	2.664	2.689	2.612
15	1.687	1.567	1.627	1.550
7.5	1.047	0.989	1.018	0.941
3.75	0.563	0.543	0.553	0.476
1.8	0.339	0.324	0.332	0.255
0.9	0.206	0.196	0.201	0.124
0.45	0.145	0.139	0.142	0.065
0	0.080	0.074	0.077

ELISA Data Analysis

Troubleshooting
and FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

F Dhaffouli, N Elloumi, K Sellami, E Bahloul,Anais Brasileiros de Dermatologia,2026
D Zhang, J Chen, H Lin, M Zhou, J Hou, W Sun,Journal of Advanced Research,2025
F Dhaffouli, N Elloumi, K Sellami, E Bahloul, S Tahri,research square,2025
Roberto Lande,Nature Communications,2019
Atri Ta.et al,Cell Signal. ,2017
Magnusson M K, Strid H, Sapnara M, et al. ,J Crohns Colitis.,2016

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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■ Q&A

we are interested in measurements of the Cathelicidin antimicrobial peptide (CAMP) in human fecal samples. According to your homepage, your assay is suitable for blood samples or tissue homogenates. But have you ever tested it in fecal samples? Would you expect it to work using fecal samples?

Thanks for your inquiry!
CSB-EL004476HU
We tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test human fecal samplee ,we suggest you do a pretest . Pls find following sample preparation method.
Feces Dilute samples with appropriate Sample Diluent. This can be achieved by adding 0.1g sample to appropriate Sample Diluent. Remove...