General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.38pg/mL |
| Detection Limit: | 15.63~1000pg/mL |
| Short Description: | This CCL11 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | CCL11 |
| Gene ID: | 6356 |
| Uniprot ID: | CCL11_HUMAN |
| Specificity: | This kit recognizes Human ECF/CCL11 in samples. No significant cross-reactivity or interference between Human ECF/CCL11 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Function | In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. |
| Protein Name | EotaxinC-C Motif Chemokine 11Eosinophil Chemotactic ProteinSmall-Inducible Cytokine A11 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-6785807 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Eotaxin ELISA kitC-C Motif Chemokine 11 ELISA kitEosinophil Chemotactic Protein ELISA kitSmall-Inducible Cytokine A11 ELISA kitCCL11 ELISA kitSCYA11 ELISA kit |
| Specificity | This kit recognizes Human ECF/CCL11 in samples. No significant cross-reactivity or interference between Human ECF/CCL11 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 96-108 | 101 |
| EDTA plasma (n=8) | 85-97 | 91 |
| Cell culture media (n=8) | 91-106 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.30 | 124.70 | 336.30 | 46.80 | 127.70 | 341.40 |
| Standard deviation | 2.60 | 5.10 | 10.10 | 2.90 | 6.40 | 16.40 |
| CV (%) | 5.07 | 4.09 | 3.00 | 6.20 | 5.01 | 4.80 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance