Human CXCL8 (Interleukin-8) Sandwich ELISA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 6 months. Following first use any unused wells and reagents should be stored according to the components table in the manual.
Assay Time:	1.5h
Detection:	Colormetric

Gene Symbol:	CXCL8
Gene ID:	3576
Uniprot ID:	IL8_HUMAN
Specificity:	This kit recognizes Human IL8 in samples. No significant cross-reactivity or interference between Human IL8 and analogues was observed.
Sample Type:	Serum and plasma

Function	Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways.
Protein Name	Interleukin-8 Il-8 C-X-C Motif Chemokine 8 Chemokine C-X-C Motif Ligand 8 Emoctakin Granulocyte Chemotactic Protein 1 Gcp-1 Monocyte-Derived Neutrophil Chemotactic Factor Mdncf Monocyte-Derived Neutrophil-Activating Peptide Monap Neutrophil-Activating Protein 1 Nap-1 Protein 3-10c T-Cell Chemotactic Factor Cleaved Into - Mdncf-A Gcp/Il-8 Protein Iv Il8/Nap1 Form I - Interleukin-8 (Ala-Il-877 Gcp/Il-8 Protein Ii Il-8(1-77 Il8/Nap1 Form Ii Mdncf-B - Il-8(5-77 - Il-8(6-77 (Ser-Il-872 Gcp/Il-8 Protein I Il8/Nap1 Form Iii Lymphocyte-Derived Neutrophil-Activating Factor Lynap Mdncf-C Neutrophil-Activating Factor Naf - Il-8(7-77 Gcp/Il-8 Protein V Il8/Nap1 Form Iv - Il-8(8-77 Gcp/Il-8 Protein Vi Il8/Nap1 Form V - Il-8(9-77 Gcp/Il-8 Protein Iii Il8/Nap1 Form Vi
Database Links	Reactome: R-HSA-2559582 Reactome: R-HSA-375276 Reactome: R-HSA-380108 Reactome: R-HSA-380994 Reactome: R-HSA-418594 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807
Cellular Localisation	Secreted
Alternative ELISA Names	Interleukin-8 ELISA kit Il-8 ELISA kit C-X-C Motif Chemokine 8 ELISA kit Chemokine ELISA kit C-X-C Motif Ligand 8 ELISA kit Emoctakin ELISA kit Granulocyte Chemotactic Protein 1 ELISA kit Gcp-1 ELISA kit Monocyte-Derived Neutrophil Chemotactic Factor ELISA kit Mdncf ELISA kit Monocyte-Derived Neutrophil-Activating Peptide ELISA kit Monap ELISA kit Neutrophil-Activating Protein 1 ELISA kit Nap-1 ELISA kit Protein 3-10c ELISA kit T-Cell Chemotactic Factor Cleaved Into - Mdncf-A ELISA kit Gcp/Il-8 Protein Iv ELISA kit Il8/Nap1 Form I - Interleukin-8 ELISA kit (Ala-Il-877 ELISA kit Gcp/Il-8 Protein Ii ELISA kit Il-8(1-77 ELISA kit Il8/Nap1 Form Ii ELISA kit Mdncf-B - Il-8(5-77 - Il-8(6-77 ELISA kit (Ser-Il-872 ELISA kit Gcp/Il-8 Protein I ELISA kit Il8/Nap1 Form Iii ELISA kit Lymphocyte-Derived Neutrophil-Activating Factor ELISA kit Lynap ELISA kit Mdncf-C ELISA kit Neutrophil-Activating Factor ELISA kit Naf - Il-8(7-77 ELISA kit Gcp/Il-8 Protein V ELISA kit Il8/Nap1 Form Iv - Il-8(8-77 ELISA kit Gcp/Il-8 Protein Vi ELISA kit Il8/Nap1 Form V - Il-8(9-77 ELISA kit Gcp/Il-8 Protein Iii ELISA kit Il8/Nap1 Form Vi ELISA kit CXCL8 ELISA kit IL8 ELISA kit
Specificity	This kit recognizes IL-8 in samples. No significant cross-reactivity or interference between IL-8 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab (100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃ (Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL96T*5: 5 vials, 14 mL
Concentrated Wash Buffer (25×)	96T/48T/24T: 1 vial, 30 mL96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL	2-8℃ (Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-103	97
EDTA plasma(n=8)	86-97	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	54.94	152.77	425.52	55.10	154.08	436.69
Standard deviation	3.28	6.97	16.13	3.07	8.67	14.89
CV (%)	5.97	4.56	3.79	5.58	5.63	3.41

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance