RayBio® G-Series Human Cytokine Antibody Array 5 Kit. Detects 80 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array G5
Human Cytokine Array G5
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 80 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability |
|
| More Information | Suggested Application Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| BDNF
| BLC (CXCL13)
| CK beta 8-1 (CCL23)
| EGF
|
ENA-78 (CXCL5)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| FGF-4
|
FGF-6
| FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
|
GCP-2 (CXCL6)
| GCSF
| GDNF
| GM-CSF
| GRO alpha (CXCL1)
|
GRO alpha/beta/gamma
| HGF
| I-309 (TCA-3/CCL1)
| IFN-gamma
| IGF-1
|
IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IL-1 alpha (IL-1 F1)
|
IL-1 beta (IL-1 F2)
| IL-2
| IL-3
| IL-4
| IL-5
|
IL-6
| IL-7
| IL-8 (CXCL8)
| IL-10
| IL-12 p40/p70
|
IL-13
| IL-15
| IL-16
| IP-10 (CXCL10)
| Leptin
|
LIF
| LIGHT (TNFSF14)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
|
MCP-4 (CCL13)
| M-CSF
| MDC (CCL22)
| MIF
| MIG (CXCL9)
|
MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
| MIP-3 alpha (CCL20)
| NAP-2 (PPBP/CXCL7)
| NT-3
|
NT-4
| Oncostatin M
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
PDGF-BB
| PLGF
| RANTES (CCL5)
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
TARC (CCL17)
| TGF beta 1
| TGF beta 2
| TGF beta 3
| Thrombopoietin (TPO)
|
TIMP-1
| TIMP-2
| TNF alpha
| TNF beta (TNFSF1B)
| VEGF-A
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array G5 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAH-CYT-G5-4.gal)
GAL (GAL-AAH-CYT-G5-8.gal)
Excel (AAH-CYT-G5_Analysis_Tool.xls)
citations
OXTRHigh stroma fibroblasts control the invasion pattern of oral squamous cell carcinoma via ERK5 signaling
- Species:
- Human
- Sample Type:
- cancer-associated fibroblasts (CAFs)
- Year:
- 2022
- Journal Name:
- Nature Communications
Lnc-PKNOX1-1 inhibits tumor progression in cutaneous malignant melanoma by regulating NF-κB/IL-8 axis
- Species:
- Human
- Sample Type:
- A2058 cell
- Year:
- 2023
- Journal Name:
- Carcinogenesis
Investigation of proliferation and migration of tongue squamous cell carcinoma promoted by three chemokines, MIP-3α, MIP-1β, and IP-10
- Species:
- Human
- Sample Type:
- CAL-27 cell, UM-1 cell, TCA-8113 cell, human oral mucosa epithelial cell
- Year:
- 2023
- Journal Name:
- OncoTargets and therapy
Inhibition of autophagy in macrophage promotes IL-1β-mediated hepatocellular carcinoma progression via inflammasome accumulation and self-recruitment
- Species:
- Human
- Sample Type:
- Huh7 cell, MHCC97-H cell
- Year:
- 2023
- Journal Name:
- Biomedicine & Pharmacotherapy
Glycogen accumulation in adipocyte precursors from elderly and obese subjects triggers inflammation via SIRT1/6 signaling
- Species:
- Human
- Sample Type:
- human adipose-derived mesenchymal stromal cells (hASCs)
- Year:
- 2022
- Journal Name:
- Aging Cell
ENA-78 Is a Novel Predictor of Wound Healing in Patients with Diabetic Foot Ulcers
- Species:
- Human
- Sample Type:
- Plasma (Wound healing fluid in diabetic patients); Wound Fluid (Wound healing fluid in diabetic patients)
- Year:
- 2019
- Journal Name:
- Journal of diabetes research
Characterization of sulfur mustard resistant keratinocyte cell line HaCaT/SM
- Species:
- Human
- Sample Type:
- Conditioned Media (HaCaT keratinocyte cell line treated with sulfur mustard)
- Year:
- 2016
- Journal Name:
- Toxicology Letters
- PubMed ID:
- 26456177
A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes
- Species:
- Human
- Sample Type:
- Cell Lysate (CD4 t cells transfected with pir30840, an IL-4 intereacting RNA)
- Year:
- 2015
- Journal Name:
- Nucleic Acids Research
- PubMed ID:
- 26405199
Leptin and Neutrophil-Activating Peptide 2 Promote Mesenchymal Stem Cell Senescence Through Activation of the Phosphatidylinositol 3-Kinase/Akt Pathway in Patients With Systemic Lupus Erythematosus
- Species:
- Human
- Sample Type:
- Serum (MSC senescence in Systemic Lupus Erthematosus (SLE))
- Year:
- 2015
- Journal Name:
- Arthritis and Rheumatology
- PubMed ID:
- 25989537
In vitro characterization of human hair follicle dermal sheath mesenchymal stromal cells and their potential in enhancing diabetic wound healing
- Species:
- Human
- Sample Type:
- Conditioned Media (Skin fibroblasts, dermal sheath MSCs)
- Year:
- 2015
- Journal Name:
- Cytotherapy
- PubMed ID:
- 25981558