Quantibody® Human Cytokine Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types.
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Human Cytokine Array Q1
Human Cytokine Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| GRO alpha/beta/gamma
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-10
| IL-12 p70
| IL-13
| IL-2
| IL-4
|
IL-5
| IL-6
| IL-8 (CXCL8)
| MCP-1 (CCL2)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MMP-9
| RANTES (CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-CYT-1.gal)
Excel (QAH-CYT-1_Analysis_Tool.xls)
citations
Extracellular Vesicles From Mesenchymal Stromal Cells Drive Muscle and Neuronal Regeneration Through TNFα Modulation
- Species:
- Human
- Sample Type:
- Other / Unspecified (protein extracts from recellularised human muscle ECM)
- Year:
- 2026
- Journal Name:
- Journal of Extracellular Vesicles
- PubMed ID:
- 41811203
Clinical efficacy and multi-omics analysis of Si-Ni-San for depression treatment in breast cancer patients: a randomized, double-blind, placebo-controlled, crossover trial
- Species:
- Human
- Sample Type:
- Whole Blood (breast cancer patients with depression)
- Year:
- 2026
- Journal Name:
- Chinese Medicine
- PubMed ID:
- 41491244
MMP28 recruits M2-type tumor-associated macrophages through MAPK/JNK signaling pathway-dependent cytokine secretion to promote the malignant progression of pancreatic cancer
- Year:
- 2025
- Journal Name:
- Journal of Experimental & Clinical Cancer Research
- PubMed ID:
- 39972459
Modulating immune toxicity in ulcerative colitis using a small molecule RAB27A inhibitor
- Year:
- 2026
- Journal Name:
- Inflammatory Bowel Diseases
- PubMed ID:
- 42627211
Aberrant Activation of the Hedgehog Pathway in Cutaneous Melanoma: Therapeutic Potential of Pharmacological Inhibitors
- Year:
- 2026
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 41596411
Suppressed basal mitophagy drives cellular aging phenotypes that can be reversed by a p62-targeting small molecule
- Year:
- 2024
- Journal Name:
- Developmental Cell
- PubMed ID:
- 38897197
Longevity-Associated Transcription Factor ATF7 Promotes Healthspan by Suppressing Cellular Senescence and Systematic Inflammation
- Species:
- Human
- Sample Type:
- Human dermal fibroblasts (HDFs)
- Year:
- 2023
- Journal Name:
- Aging and disease
Radiation Exposure Induced Blood-Brain Barrier Injury via Mitochondria-Mediated Sterile Inflammation
- Year:
- 2025
- Journal Name:
- Advanced science (Weinheim, Baden-Württemberg, Germany)
- PubMed ID:
- 40433769
Real-Time Exposure to 3D-Printing Emissions Elicits Metabolic and Pro-Inflammatory Responses in Human Airway Epithelial Cells
- Year:
- 2024
- Journal Name:
- Toxics
- PubMed ID:
- 38251022
Case study: May human norovirus infection be associated with premature delivery?
- Species:
- Human
- Sample Type:
- Tissue Lysate / Homogenate (placenta, fetal membrane); Other Body Fluid (amniotic fluid); Whole Blood (umbilical cord blood)
- Year:
- 2024
- Journal Name:
- Virologica Sinica
- PubMed ID:
- 39490793