Human kappa (kappa chain) antibody (Peroxidase)

Cat# orb347227-1mg

Size : 1mg

Brand : Biorbyt

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    Human kappa (kappa chain) antibody (Peroxidase)

    Human kappa (kappa chain) antibody (Peroxidase)

    Catalog Number: orb347227

    Catalog Numberorb347227
    CategoryAntibodies
    DescriptionHuman kappa (kappa chain) antibody (Peroxidase)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityHuman
    IsotypeIgG
    ImmunogenAnti-Human kappa chain was produced by repeated immunization with human kappa light chain in goat.
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:77,000, IHC: 1:500 - 1:2,000, WB: 1:1,000 - 1:10,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
    ConjugationHRP
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Thimerosal
    Alternative namesGoat Anti-Human κ (kappa chain) Antibody Peroxidas
    NoteFor research use only
    Application notesAnti-Human k (kappa chain) Antibody has been tested by ELISA and is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-human kappa chain Antibody has been assayed against 1.0 ug of human kappa light chain in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.
    Expiration Date12 months from date of receipt.