Human MMP11 (Stromelysin-3) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	MMP11
Gene ID:	4320
Uniprot ID:	MMP11_HUMAN
Specificity:	This kit recognizes Human MMP-11 in samples. No significant cross-reactivity or interference between Human MMP-11 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Specifically expressed in stromal cells of breast carcinomas.
Post Translational Modifications	The precursor is cleaved by a furin endopeptidase.
Function	May play an important role in the progression of epithelial malignancies.
Protein Name	Stromelysin-3 Sl-3 St3 Matrix Metalloproteinase-11 Mmp-11
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1474228 Reactome: R-HSA-1592389
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative CLIA Names	Stromelysin-3 CLIA kit Sl-3 CLIA kit St3 CLIA kit Matrix Metalloproteinase-11 CLIA kit Mmp-11 CLIA kit MMP11 CLIA kit STMY3 CLIA kit
Specificity	This kit recognizes Human MMP-11 in samples. No significant cross-reactivity or interference between Human MMP-11 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	96-111	104
EDTA plasma(n=8)	88-102	94
Cell culture media(n=8)	100-113	107

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	110.56	282.34	841.22	116.47	301.58	826.08
Standard deviation	12.25	32.84	82.27	9.67	35.19	65.34
CV (%)	11.08	11.63	9.78	8.30	11.67	7.91

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance