Human MUC4 (Mucin-4) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	MUC4
Gene ID:	4585
Uniprot ID:	MUC4_HUMAN
Specificity:	This kit recognizes Human MUC4 in samples. No significant cross-reactivity or interference between Human MUC4 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
Post Translational Modifications	Proteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain. Mucin-4 alpha chain: Highly O-glycosylated. Mucin-4 beta chain: Is predominantly N-glycosylated.
Function	Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces. These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation. Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.
Protein Name	Mucin-4 Muc-4 Ascites Sialoglycoprotein Asgp Pancreatic Adenocarcinoma Mucin Testis Mucin Tracheobronchial Mucin Cleaved Into - Mucin-4 Alpha Chain Ascites Sialoglycoprotein 1 Asgp-1 - Mucin-4 Beta Chain Ascites Sialoglycoprotein 2 Asgp-2
Database Links	Reactome: R-HSA-5083625 Reactome: R-HSA-5083632 Reactome: R-HSA-5083636 Reactome: R-HSA-5621480 Reactome: R-HSA-913709 Reactome: R-HSA-977068
Cellular Localisation	Mucin-4 Beta Chain: Cell Membrane Single-Pass Membrane Protein Isoforms Lacking The Cys-Rich Region Egf-Like Domains And Transmembrane Region Are Secreted Secretion Occurs By Splicing Or Proteolytic Processing Mucin-4 Alpha Chain: Cell Membrane Secreted Forms A Complex With Mucin-4 Beta Chain At The Cell Membrane Isoform 3: Cell Membrane Isoform 11: Secreted Isoform 15: Secreted Isoform 17: Cell Membrane
Alternative CLIA Names	Mucin-4 CLIA kit Muc-4 CLIA kit Ascites Sialoglycoprotein CLIA kit Asgp CLIA kit Pancreatic Adenocarcinoma Mucin CLIA kit Testis Mucin CLIA kit Tracheobronchial Mucin Cleaved Into - Mucin-4 Alpha Chain CLIA kit Ascites Sialoglycoprotein 1 CLIA kit Asgp-1 - Mucin-4 Beta Chain CLIA kit Ascites Sialoglycoprotein 2 CLIA kit Asgp-2 CLIA kit MUC4 CLIA kit
Specificity	This kit recognizes Human MUC4 in samples. No significant cross-reactivity or interference between Human MUC4 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-105	98
EDTA plasma(n=8)	85-97	91
Cell culture media(n=8)	90-104	98

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	204.66	626.41	1420.50	209.12	675.43	1549.06
Standard deviation	24.29	48.11	105.69	20.79	62.27	128.73
CV (%)	11.87	7.68	7.44	9.94	9.22	8.31

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance