General Info
| Applications: | ELISA |
| Reactivity: | Human |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 0.19ng/mL |
| Detection Limit: | 0.31~20ng/mL |
| Short Description: | This MUC7 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
Information
| Gene Symbol: | MUC7 |
| Gene ID: | 4589 |
| Uniprot ID: | MUC7_HUMAN |
| Specificity: | This kit recognizes Human MUC7 in samples. No significant cross-reactivity or interference between Human MUC7 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
Description
| Post Translational Modifications | N- and O-glycosylated. Contains fucose, mannose, galactose, N-acetylglucosamine and N-acetylgalactosamine. |
| Function | May function in a protective capacity by promoting the clearance of bacteria in the oral cavity and aiding in mastication, speech, and swallowing. Binds P.aeruginosa pili. |
| Protein Name | Mucin-7Muc-7Apo-Mg2Salivary Mucin-7 |
| Database Links | Reactome: R-HSA-5083625Reactome: R-HSA-5083632Reactome: R-HSA-5083636Reactome: R-HSA-5621480Reactome: R-HSA-913709Reactome: R-HSA-977068 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Mucin-7 ELISA kitMuc-7 ELISA kitApo-Mg2 ELISA kitSalivary Mucin-7 ELISA kitMUC7 ELISA kitMG2 ELISA kit |
| Specificity | This kit recognizes Human MUC7 in samples. No significant cross-reactivity or interference between Human MUC7 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-102 | 95 |
| EDTA plasma (n=8) | 89-103 | 96 |
| Cell culture media (n=8) | 87-99 | 93 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.10 | 2.41 | 8.67 | 1.14 | 2.44 | 8.90 |
| Standard deviation | 0.06 | 0.11 | 0.36 | 0.06 | 0.14 | 0.29 |
| CV (%) | 5.45 | 4.56 | 4.15 | 5.26 | 5.74 | 3.26 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance