Quantibody® Human Th17 Array 1 Kit. Detects 20 Human Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types.
Human Th1/Th2/Th17 Array Q1
Human Th1/Th2/Th17 Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | T Helper Cells, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-10
| IL-12 p70
|
IL-13
| IL-17A
| IL-17F
| IL-2
| IL-21
|
IL-22
| IL-23
| IL-28A (IFN-lambda 2)
| IL-4
| IL-5
|
IL-6
| MIP-3 alpha (CCL20)
| TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Th1/Th2/Th17 Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-TH17-1.gal)
Excel (QAH-TH17-1_Analysis_Tool.xls)
citations
Dietary supplementation of arachidonic acid promotes humoral immunity
- Species:
- Mouse
- Sample Type:
- Serum (OVA-immunized mice, days 7 and 14)
- Year:
- 2025
- Journal Name:
- EMBO Molecular Medicine
- PubMed ID:
- 40940568
Impact of Black Soldier Fly Larvae Oil on Immunometabolic Processes
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants from PBMC treatments)
- Year:
- 2025
- Journal Name:
- International Journal of Molecular Sciences
- PubMed ID:
- 40429995
Astragaloside IV Ameliorates Inflammatory Damage in Mice with Acute Liver Failure
- Species:
- Human
- Sample Type:
- THP-1 cell
- Year:
- 2023
- Journal Name:
- Chinese medicine
Role of tenofovir dipivoxil in gut microbiota recovery from HBV-infection induced dysbiosis
- Year:
- 2024
- Journal Name:
- BMC Microbiology
- PubMed ID:
- 39304810
Dapagliflozin alleviates renal inflammation and protects against diabetic kidney diseases, both dependent and independent of blood glucose levels
- Species:
- Human
- Sample Type:
- HK-2 cell
- Year:
- 2023
- Journal Name:
- Frontiers in immunology
HSP22 suppresses diabetes-induced endothelial injury by inhibiting mitochondrial reactive oxygen species formation
- Species:
- Mouse
- Sample Type:
- Serum (diabetic mice)
- Year:
- 2019
- Journal Name:
- Redox Biology
- PubMed ID:
- 30640127
Isoforskolin and forskolin attenuate lipopolysaccharide-induced inflammation through TLR4/MyD88/NF-B cascades in human mononuclear leukocytes
- Species:
- Human
- Sample Type:
- Conditioned Media (mononuclear leukocytes treated with various isoforskolin and forskolin treatments)
- Year:
- 2019
- Journal Name:
- Phytotherapy Research
- PubMed ID:
- 30637903
LR12 Promotes Liver Repair by Improving the Resolution of Inflammation and Liver Regeneration in Mice with Thioacetamide- (TAA-) Induced Acute Liver Failure
- Species:
- Human
- Sample Type:
- Cell Culture Supernatant (supernatants from macrophages control, TAA, LR12, TAA+LR12)
- Year:
- 2021
- Journal Name:
- Mediators of Inflammation
- PubMed ID:
- 34135689
Herpes simplex virus type I-infected disorders alter the balance between Treg and Th17 cells in recurrent herpes labialis patients
- Species:
- Human
- Sample Type:
- Serum (recurrent herpes labialis vs healthy)
- Year:
- 2020
- Journal Name:
- International journal of immunopathology and pharmacology
- PubMed ID:
- 32735468
Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome
- Species:
- Human
- Sample Type:
- Conditioned Media ( PBMC cells)
- Year:
- 2013
