IgA, alpha (TRITC)
Cat# I1890-05U-500ug
Size : 500ug
Brand : US Biological
Host
rabbitConjugate
TRITCIsotype
IgGGrade
Affinity PurifiedApplications
E IF WBShipping Temp
Blue IceStorage Temp
-20°CApplications:
Suitable for use in Immunofluorescence Microscopy, Fluorescent Western Blot and FLISA. Other applications not tested.
Recommended Dilutions:
Immunofluorescence Microscopy: 1:1000-1:5000
FLISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
FLISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength:
550nm
Emission Wavelength:
570nm
Fluorochrome/Protein Ratio:
2.5 moles TRITC per mole of Rabbit IgG
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Product Type
Pab
Isotype
IgG
Host
rabbit
Source
mouse
Concentration
~1mg/ml
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Tetramethylrhodamine isothiocyanante (TRITC) (MW 444D).
Purity
Purified by immunoaffinity chromatography.
Immunogen
Mouse IgA alpha chain
Specificity
Recognizes mouse IgA. Assay by IEP resulted in a single precipitin arc against anti-rabbit serum, mouse IgA and mouse serum. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy or light chain isotypes..
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.