IgG, Kappa (AP)

Suitable for use in Immunoblot (Western or Dot Blot), ELISA and immunohistochemistry. Other applications not tested.

ELISA (Capture): 1:1000-1:2000. Assayed against 1ug of mouse IgG.
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Alkaline Phosphatase (Calf Intestine) (MW: 140,000D)

Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Pab

IgG

rabbit

mouse

~1mg/ml (UV280)

Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).

Purified by immunoaffinity chromatography.

Mouse k (kappa light chain)

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Solid phase adsorptions against a panel of mouse IgG lambda, IgM lambda and IgA lambda proteins was performed. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy or light chain isotypes.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.