Reagents and instruments for immunology, cell biology and molecular biology.

Kappa Light Chain(TB 28-2), APC conjugate, 0.1mg/mL

Print

Cat# BNCA0708-250

Size : 250uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Biotium

Contact local distributor :

Phone : +1 850 650 7790

Human Kappa Light Chain Monoclonal Mouse Antibody (TB28-2)

Product Attributes

Antibody number	#0708
Antibody reactivity (target)	Human Kappa Light Chain
Antibody type	Primary, Anti-Human Immunoglobulin
Host species	Mouse
Clonality	Monoclonal
Clone	TB28-2
Isotype	IgG1, kappa
Molecular weight	~22.5 kDa
Synonyms	HCAK1; Ig Kappa Chain C Region; IGKC; Immunoglobulin KM
Human gene symbol	IGKC
Entrez gene ID	3514
SwissProt	P01601 & P01834
Unigene	449609
Immunogen	Human IgG-kappa myeloma protein
Antibody target cellular localization	Plasma membrane, Secreted (extracellular)
Verified antibody applications	IHC (FFPE) (verified)
Species reactivity	Human
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Immunohistology formalin-fixed 0.5-1.0 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user
Positive control	293T, Raji or hPBL cells. Tonsil or Spleen
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Cell/tissue expression	B-cells
Antibody research areas	Cancer, Immunology, Inflammation
Tumor expression	Leukemia/lymphoma

Product Description

This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@clinisciences.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.