LentiTran, a transfection reagent for high titer lentivirus production (0.5 mL)
Cat# TT400001
Size : 500ul
LentiTran, a transfection reagent for high titer lentivirus production (0.5 mL)
SKU
TT400001
LentiTran, a transfection reagent for high titer lentivirus production, 0.5 mL
Citations
Tankyrase1/2 inhibitor XAV-939 reverts EMT and suggests that PARylation partially regulates aerobic activities in human hepatocytes and HepG2 cells
De Vos, Mavrogiannis, Clarinda Wolters et al
Biochem Pharmacol (2024) 227, 116445 DOI: 10.1016/j.bcp.2024.116445
De Vos, Mavrogiannis, Clarinda Wolters et al
Biochem Pharmacol (2024) 227, 116445 DOI: 10.1016/j.bcp.2024.116445
Cited by 3
AI Summary: OriGene’s Lentitran transfection reagent (TT400001) was used to generate lentiviral vectors in HEK293T cells, enabling efficient genetic manipulation of HepG2 cells in the study.
Snippet: To make the lentiviral vectors, HEK293T cells were transiently transfected with packaging plasmids (ps-PAX2 and ps-PMD2.g) and one of the main plasmids, as shown in Table 4 , using Lentitran (TT400001, OriGene, Rockville, USA). After 48 h and 72 h, medium was collected from the HEK293T cells and filtered through a 0.45 μm filter (SLHV033RB, Millipore). …
Overexpression of G3BP1 facilitates the progression of colon cancer by activating β‑catenin signaling
Li, Wang, Zhong et al
Mol Med Rep (2020) 22 (5), 4403-4411 DOI: 10.3892/mmr.2020.11527
Li, Wang, Zhong et al
Mol Med Rep (2020) 22 (5), 4403-4411 DOI: 10.3892/mmr.2020.11527
Cited by 18
AI Summary: OriGene's short hairpin RNA (shRNA) lentivirus vector targeting β-catenin was used to silence β-catenin expression in colon cancer cells, enabling the authors to demonstrate the role of β-catenin in G3BP1-mediated colon cancer progression.
Snippet: To silence β-catenin expression, colon cancer cells were infected with the lentivirus vector [short hairpin RNA (sh)-β-catenin;OriGene Technologies, Inc.] and puromycin (100 µg/ml) was applied to select the stably infected cells at 37°C for 14 days;- To silence β‑catenin expression, colon cancer cells were infected with the lentivirus vector [short hairpin rna (sh)‑β‑catenin;OriGene Technologie...
Analysis of genomic pathogenesis according to the revised Bethesda guidelines and additional criteria
Kim, Kim, Ha et al
J. Cancer Res. Clin. Oncol. (2020)
Kim, Kim, Ha et al
J. Cancer Res. Clin. Oncol. (2020)
Cited by 8
AI Summary: OriGene’s DDK-tagged pCMV6-entry vector was used to subclone wild-type and mutant cDNAs of SH2D4A, KRTAP27-1, and NUP160 for stable transfection in cell-based functional assays, enabling analysis of their roles in colorectal cancer pathogenesis.
Snippet: … Both wild- and mutant-type cDNAs of three genes, ie, SH2D4A, KRTAP27-1, and NUP160, were amplified by PCR and subcloned into DDK-tagged pCMV6-entry for stable transfection (OriGene, Rockville, MD, USA);- knockdown. Both wild- and mutant-type cDNAs of three genes, ie, SH2D4A, KRTAP27-1, and NUP160, were amplified by PCR and subcloned into DDK-tagged pCMV6-entry for stable transfection (OriGene, Rockville, MD, USA)
Interleukin 21 Receptor/Ligand Interaction Is Linked to Disease Progression in Pancreatic Cancer
Linnebacher, Mayer, Marnet et al
Cells (2019) 8 (9) DOI: 10.3390/cells8091104
Linnebacher, Mayer, Marnet et al
Cells (2019) 8 (9) DOI: 10.3390/cells8091104
AI Summary: In this study, OriGene products—including the universal scrambled control siRNA (SR30004) and PRDM1-targeting siRNAs (SR300437A)—were used for siRNA-mediated gene knockdown experiments to investigate the role of Blimp-1 (PRDM1) in pancreatic cancer cell invasion and response to IL-21.
Snippet: Transient transfection (for PRDM1 siRNA knockdown): Pancreatic cancer cells BxPC-3 and Panc-1 in a 6-well plate were transfected with 10 nM of a universal scrambled control siRNA (SR30004;OriGene) or three individual PRDM1-targeting siRNAs (SR300437A Cached
Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGFβ
Ren, Zheng, Bommarito et al
Sci Rep (2019) 9 (1), 6688 DOI: 10.1038/s41598-019-43239-x
Ren, Zheng, Bommarito et al
Sci Rep (2019) 9 (1), 6688 DOI: 10.1038/s41598-019-43239-x
AI Summary: OriGene products contributed to this study by providing lentiviral NOS shRNA vectors for knockdown of NOS isoforms and a scramble control vector (CAT No: TR30021), enabling the authors to investigate the role of nitric oxide synthase in precancerous mammary lesion formation.
Snippet: To knock-down each NOS isoform, lentiviral NOS shRNA vectors that target respective sequences were obtained from Origene (Supplementary Table 1). As a control, lentiviral vector that expresses scramble sequence (CAT No: TR30021) was used
RNA Binding Antagonizes Neurotoxic Phase Transitions of TDP-43
Mann, Gleixner, Mauna et al
Neuron (2019) 102 (2), 321-338.e8 DOI: 10.1016/j.neuron.2019.01.048
Mann, Gleixner, Mauna et al
Neuron (2019) 102 (2), 321-338.e8 DOI: 10.1016/j.neuron.2019.01.048
AI Summary: OriGene’s One-Wash™ Lentivirus Titer Kit (p24 ELISA, Cat# TR30038) was used in this study to quantify lentiviral particles, facilitating efficient viral transduction for investigating TDP-43 proteinopathy mechanisms.
Snippet: Critical Commercial Assays Pierce BCA Protein Assay Thermo Fisher Scientific Cat# 23225 RC DC Protein Assay Bio-Rad Cat# 5000122 NE-PER Nuclear and Cytoplasmic Extraction Reagents Thermo Fisher Scientific Cat# 78833 One-Wash Lentivirus Titer Kit, p24 ELISA OriGene Cat# TR30038 CellTiter-Glo Luminescent Cell Viability Assay Promega Cat# G7572
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