This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Methionine-Enkephalin(MEK) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Methionine-Enkephalin(MEK). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionine-Enkephalin(MEK) in the samples is then determined by comparing the OD of the samples to the standard curve.
Reactivity
All
Range
125.00-8000 pg/mL
Sensitivity
75.00 pg/mL
Protein Length
2h
Alternative names
Met-enkephalin, Opioid growth factor, OGF
Note
For research use only
Sample Types
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids